Despite several advantages, ELISA tests have been reported to have lower sensitivity than neutralization tests
CHIKV, chikungunya virus; dpo, days post-symptom onset; E, envelope; NS, nonstructural protein; PRNT, plaque reduction neutralization test
* Positive result from immunoglobulin M antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) with confirmatory plaque reduction neutralization test
The anti-FMD "O" antibody titer in serum of the vaccinated animals is determined using complement fixation test and virus neutralization test
(Grist et al., 1979; Doel, 1999; Iyer et al., 2000 and Doel, 2003).
When interpreting WR samples, one should look at the whole profile of HBV tests, including the HBsAg neutralization test
and assays of other hepatitis serologic markers, to provide the most accurate status of HBV infection to clinicians.
Of 10 patients sampled, 8 seroconverted after 2 or 3 serial bleeds, as evidenced by a 4-fold increase in the hemagglutination inhibition assay or virus neutralization test
titers (Appendix Table 1), and had RVFV-specific IgM, confirming their recent RVFV infection status.
Plaque-reduction neutralization tests
(3) indicated that patient A had been infected with Zika virus, dengue virus serotype 1, or both, but that patient B had been infected only with Zika virus.
Red indicates roe deer serum samples that showed positive results in the TBEV neutralization test
, and blue indicates roe deer serum samples that showed negative results in this test or an ELISA.
We tested all 234 samples for Zika virus neutralizing antibody by 50% plaque reduction neutralization test
([PRNT.sub.50]) on Vero cells (Appendix, https://wwwnc.cdc.gov/EID/article/25/2/18-0258-App1.
Samples testing positive for YFV-specific IgM antibodies were evaluated using the plaque reduction neutralization test
, with a 90% cutoff value for neutralizing antibody titers against YFV (the standard evaluation at CDC for determining serologic response to YF vaccine virus).
Samples suspected to be Zika virus positive were those that were positive on initial Zika virus [PRNT.sub.90] (plaque reduction neutralization test
with neutralization defined as [greater than or equal to] 90% reduction in challenge virus PFUs) screening when using a 1:10 serum sample dilution.
Umbilical cord serum and maternal serum were tested for LACV-specific antibodies by ELISA and serum-dilution plaque-reduction neutralization test