The human CoV-EMC/2012 strain was used for 50% tissue culture infectious dose assays, microneutralization assays, and plaque-reduction neutralization tests
However, serum samples drawn 26-37 days posttransfusion indicated that three of the four recipients had YFV-IgM antibodies confirmed by plaque reduction neutralization test
We adapted the previously described focus-reduction neutralization test
(FRNT) (28) to a 96-well format (18).
Dpi, day postinfection for challenge (initial infection); MFI, median fluorescent intensity; VNT, virus neutralization test
To ensure that diagnostic criteria of acute infection were met, as previously described, IgM-positive samples were confirmed with IgG IFA testing (5) and studied with INKV prototype strain KN3641 (1), CHATV Mohko strain M07-1 (13), and TAHV prototype strain Bardos 92 (28) plaque-reduction neutralization test
Zika virus-specific plaque reduction neutralization test
results were positive on convalescent-phase serum samples.
Serum samples from 36 patients from 2006 through 2008 were typed by focus reduction neutralization test
with Hantaan virus (HTNV) strain 76-128 and Seoul virus (SEOV) strain UR in Vero cells.
Delayed antibody responses with the neutralization test
were associated with more severe disease.
The 50% plaque-reduction neutralization test
showed that the father, mother, and 14-year-old daughter had neutralizing antibodies against orthopoxvirus (titers 800, 3,200, and 800 neutralizing units/mL, respectively).
The viruses were stored at -80[degrees]C until the neutralization test
Banked blood samples collected from white-tailed deer, raccoon, and (occasionally) moose (Alces alces) and coyote (Canis latrans) during 2009-2014 were analyzed by plaque-reduction neutralization test
for HRTV neutralizing antibodies by using African green monkey kidney (Vero) cell culture.
We used a 90% plaque-reduction neutralization test