The neutral red retention assay used here followed a hybridized version of the protocols described by Lowe et al.
A neutral red stock solution was prepared by dissolving 20 mg neutral red (Sigma) in 1 mL DMSO (99.
Abbreviations: FFD, four flavonoids from Daphne genkwa: FCA, Freund's complete adjuvant; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NO, nitric oxide; NRU, neutral red
uptake; LPS, lipopolysaccharide; DPPH, 2,2diphenyl-1-picrylhydrazyl; DXM, dexamethasone; TGP, total glucosides of paeony; RA, rheumatoid arthritis; AA, adjuvant arthritis; PG, prostaglandins; DMEM, Dulbecco's Modified Eagle's Medium; iNOS, nitrite oxidative synthase.
After 2 wk of acclimatization, the bivalves were bled for neutral red staining.
0 g commercial neutral red from Sigma-Aldrich (Castle Hill, NSW) in 100 mL distilled water.
2] did not result in a decrease in neutral red
uptake compared with control.
The cellular toxicity of the seed extract of Licania tomentosa was verified by morphological changes in HEp-2 cell cultures and the determination of cell viability by neutral red
KEY WORDS: neutral red
, lysosomal membrane stability, stress, sexual maturation, spawning, Pacific oysters, Crassostrea gigas
This research expands on the toxicologic profile of organic compounds adsorbed to particulate matter, specifically in Puerto Rico, by using the cytotoxic neutral red
ABSTRACT Neutral red
retention (NRR) assay was used to evaluate the effect of changes in water temperature on lysosomal membrane integrity in the hemolymph of blacklip abalone, Haliotis rubra.
The neutral red
assay (NRA) was evaluated as an indicator of stress response in mussels that were held under various culture situations.
Further, it is a direct replacement for MTT, XTT, Neutral Red
and other research indicators to determine cell viability.