At 10 g/ml concentration cells were totally round and they also didn't uptake neutral red
and they were appearing transparent in color instead of red (Fig.
The Neutral red
retention (NRR) assay used in this study was modified from the methods used by Lowe et al.
After exposure time, the extracts from particulate matter were decanted and a neutral red
dye solution of 100 [micro]g/mL was added to all wells, with the exception of the blanks.
In their stable form (no stress response), lysosomes will accumulate and retain the neutral red
dye for an extended period of time.
Tryptone, Neutral red
C15H17CIN4, yeast extract (Oxoid), Sodium hydrogen phosphate Na2HPO4, potassium phosphate KH2PO4, sodium chloride NaCl, and glucose C6H12O6 (Merck) H2SO4 (sigma) were used as received.
was also identified by Salih and Logan (1990) as the most promising of 30 dyes listed as markers for Microtermes lepidus Sjostedt.
The method is based on the assessment of cell viability in vitro after exposure to the AE, measuring the lysosome's uptake of neutral red
Add 100 [micro]l Neutral Red
medium Incubate (37[degrees] [+ or -] 1[degrees]C/5.
Light microscopy and neutral red
staining confirmed the presence of plaque formation with a central clearing surrounded by live (red) cells (Fig.
KEY WORDS: abalone, fishing stress, morbidity, hemolymph, Haliotis iris, sodiurmpotassium ratio, heart rate, neutral red
Briefly after measurement of NO production, the supernatant was removed from each well and replaced with neutral red
working solution, following incubation for 30 min.
Using the Neutral Red
Assay as a measure of physiological stress response, Harding et al.