Because none of these CSG viruses caused disease in older mice after intraperitoneal inoculation, adult and aged mice were inoculated intranasally with each virus to determine if they were neurovirulent (i.e., could replicate in the brain) and neuropathogenic (i.e., could cause disease in the brain) after a more direct inoculation route and to determine if neuropathogenesis
was restricted by age.
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