(A) Positive neurofilament
labeling of neuroectodermal fibers (neurofilament
immunohistochemical stain; bar = 100 nm).
light chain in cerebrospinal fluid and prediction of disease activity in clinically isolated syndrome and relapsing-remitting multiple sclerosis.
The HCs, presynaptic ribbons, and nerve fibers were identified by immunostaining for myosin 7a (red), Ctbp2 (magenta), and neurofilament
(NF) (green), respectively.
The results of immune staining for S-100 and neurofilament
showed various outcomes.
C and D, Glial and neuronal components of the outbursts (dashed line with arrowhead), respectively, with a bundle of axons in D (asterisk) (hematoxylin-eosin, original magnifications, X8 [A] and X200 [B]; GFAP [glial fibrillary acidic protein; clone 6F2, DakoCytomation, Glostrup, Denmark], original magnification X300 [C]; nonphosphorylated neurofilament
[SMI32, Covance, Princeton, New Jersey] original magnification, X300 [D]).
In ganglion-like cells, CD56 showed the highest positive rate (100%, 19/19), followed by synaptophysin (98.4%, 61/62), NSE (87.0%, 80/92), somatostatin (51.3%, 41/80), Bcl2 (36.4%, 4/11), pancreatic polypeptide (32.1%, 27/84), chromogranin A (28.9%, 28/97), neurofilament
(27.3%, 18/66), vimentin (25.0%, 1/4), and S-100 (23.9%, 28/117).
We also studied the cytoskeletal integrity of neurons, by observing the expression of neurofilament
protein (NF200) in the spinal cord.
The image scale was first set and converted to 8 bit and binary, which will convert the green fluorescence (neurofilament
staining) into a black image with a white background.
Stigbrand, "Elevated neurofilament
levels in neurological diseases," Brain Research, vol.
The characters of PC12 cells are very similar to sympathetic neuron system, that is, the characters of neurofilament
(NF) expression and neurite outgrowth in responding to challenge of nerve growth factor (NGF).
Caption: Figure 5: The results of NF-200 staining showed the regenerated neurofilament
in all three groups of grafts.