Several studies have demonstrated that HBO could increase Nestin
expression in SVZ and hippocampus, which is involved in the repair of injured nerves.,, We found that combination therapy enhanced Nestin
expression in SVZ when compared to antivenom alone, especially in the early period.
More than 97% of the cultured aNSCs were positive for NSC markers Nestin
and Sox2 (Supplemental Figures 1(a)-(d)), suggesting the homogeneity of aNSCs.
In our previous study, we detected reexpression of nestin
in the contralateral motor cortex only in injured rats treated with GH.
Immunostaining of cells plated for 24 h revealed that > 99% were positive for the stem cell microfilament protein nestin
(not shown), and that nearly all cells expressed the low affinity nerve growth factor receptor p75 (Fig.
Cytokeratin 19 (CK19, BA2266) and nestin
(BA1289) antibodies were from Boster Biological (Wuhan, China).
(10) This approach may be further refined using the novel markers nestin
and inositol phosphate 4-phosphatase.
Secondary antibodies were as follows: Alexa Fluor 488 for BrdU label and Alexa Fluor 594 for nestin
label (Invitrogen[TM], Thermo Fisher Scientific Inc., 1:1000).
Using confocal microscopy and RT-PCR they demonstrated the rare co-localization of nestin
and insulin, both in freshly isolated islets and in long-term cultures enriched with nestin-positive cells obtained from cadaveric donors.
The tumours were variably positive for nestin
(78.8%; 26/33) and GFAP (63.6%; 21/33), three tumours had CD34+ cells, but all were negative for CD117, alpha-smooth muscle actin and desmin.
Microvessel proliferation by co-expression ofendothelial nestin
and Ki-67 is associated with a basal-likephenotype and aggressive features in breast cancer.
Florian et al., "Gene expression of nestin
, collagen type I and type III in human dental follicle cells after cultivation in serum-free medium," Oral and Maxillofacial Surgery, vol.