nested PCR


Also found in: Acronyms.

nested PCR

amplification of an AMPLICON by using PRIMERS (nested primers) internal to the original primers used to generate that amplicon.
References in periodicals archive ?
gondii-specific real-time PCR (6) and nested PCR (7) (Table).
Results showed C psittaci was detected in 37 (18.5%) of 200 birds (18/37 symptomatic and 19/37 asymptomatic birds) by nested PCR assay.
Double nested PCR was carried out by Applied Biosystems 2720 Thermal Cycler in every patient to determine the isoform of PML RAR[alpha] fusion gene.
100 percent and 100 percent, respectively relative to nested PCR, when conjunctival swabs were tested.
Caption: Figure 2: Apal digestion of the 292 bp nested PCR (QPCR) fragment generated from the fragment of 1.12 RT-PCR reactions.
For nested PCR, the MBR of the t(14;18) translocation was amplified by a two-step nested PCR as previously described (10).
In one of the Brazilian study, the mycobacterial DNA was detected in 24.32% of the biopsies[6] similar to the present study's nested PCR results (25.8%).
In the first nested PCR, 4 [micro]L of DNA lysate obtained after the tissue digestion was used as a source of HPV DNA template, to amplify a 630 bp region in the E6/E7 region of the HPV genome using HPV general primers.
Nested PCR. To further amplify the target genes, Phusion Hot Start II High Fidelity DNA Polymerase kit (Life Technologies, Canada) was used for nested PCR with primers described in Table 1 [14, 15].
With the purified DNA, nested PCR was conducted to detect malaria parasites using species specific primers as described by Snounou et al.

Full browser ?