The impact of neocyte transfusion in the management of thalassemia.
Neocytes transfusion may be useful in the treatment of young patients with severe chronic anaemia (e.g.
But, previous studies show that transfusion of concentrated neocytes/pooled neocytes demonstrated reduction in numbers of required transfusion, extension of transfusion intervals, reduction of the incidences of transfusion related hazards and reduction of iron overload in patients with beta-thalassemia major.
Various techniques have been developed to collect neocytes. One approach is in the form of aphaeresis termed as neocytaphaeresis  We can prepare concentrated neocytes by centrifugation and separation technique using refrigerated centrifuge by the principle of density.
To compare the results of neocytes concentrate/pooled neocytes transfusion over conventional packed red blood cells transfusion in a group of beta-thalassemia major patients to maintain normal physiological level of haematocrit ([less than or equal to] 35%) and haemoglobin level ([less than or equal to] 11 gm/dL) as per super-transfusion regimen and with previous studies.
Six (06) patients were transfused with conventional packed red blood cells (Control group) and remaining six (06) patients were transfused with neocytes concentration/pooled neocytes (Study group) for nine (09) months (172 days) from June 11, 2010 to March 8, 2011.
In subjects exposed to conditions triggering neocytolysis, beside the dramatic reduction of young RBCs counts, changes in neocytes membrane components have been observed contributing to a "senescent-like" phenotype and likely targeting them to macrophage phagocytosis.
In subjects exposed to conditions triggering neocytolysis, beside the dramatic reduction of young red cells counts, changes in neocytes membrane components have been observed, and that contributes to a "senescent-like" phenotype and likely targets them to macrophage phagocytosis (10).
The methods used for the separation of neocytes and the analysis of their biochemical and functional features have been described in several scientific reports and appropriately changed by the researcher author of this proposal.
The neocytes separated from the whole RBCs population have been subjected to a number of analysis performed at cellular and molecular level.
2) Does HIF mediated EPO's increase, in high altitude give rise to a plethora of scarcely selected, hypoxia adapted neocytes whose phenotype makes them susceptible to phagocytosis, upon return to normoxia?
This was evaluated by comparative flow cytometry analysis of RBCs drawn before, during and after the high altitude dwelling, and the data were also supported by western blotting of partially purified hemoglobin and hemolysates of neocytes and by Q-RTPCR of m-RNA of reticulocytes enriched fractions from blood samples drawn at the three time points above mentioned (14).