If neocytes with an average 90 days lifespan are transfused instead of conventional packed red blood cells with an average 60 days lifespan (Half-life of neocyte is almost twice that of conventional red blood cell) to a beta-thalassemia major patient, then the time interval between two transfusions can be increased, incidences of transfusion related hazards and the chances of inducing haemosiderosis may be reduced by decreasing numbers of required red blood cells units transfusion as neocytes survive in the circulation longer than older cells and may reduce the total red blood cells requirementl  The inconvenience of pooled neocytes transfusion is exposure to a large number of donors.
The impact of neocyte transfusion in the management of thalassemia.
Neocytes transfusion may be useful in the treatment of young patients with severe chronic anaemia (e.
One approach is in the form of aphaeresis termed as neocytaphaeresis  We can prepare concentrated neocytes by centrifugation and separation technique using refrigerated centrifuge by the principle of density.
To compare the results of neocytes concentrate/pooled neocytes transfusion over conventional packed red blood cells transfusion in a group of beta-thalassemia major patients to maintain normal physiological level of haematocrit ([less than or equal to] 35%) and haemoglobin level ([less than or equal to] 11 gm/dL) as per super-transfusion regimen and with previous studies.
In subjects exposed to conditions triggering neocytolysis, beside the dramatic reduction of young RBCs counts, changes in neocytes membrane components have been observed contributing to a "senescent-like" phenotype and likely targeting them to macrophage phagocytosis.
In subjects exposed to conditions triggering neocytolysis, beside the dramatic reduction of young red cells counts, changes in neocytes membrane components have been observed, and that contributes to a "senescent-like" phenotype and likely targets them to macrophage phagocytosis (10).
The methods used for the separation of neocytes and the analysis of their biochemical and functional features have been described in several scientific reports and appropriately changed by the researcher author of this proposal.
The neocytes separated from the whole RBCs population have been subjected to a number of analysis performed at cellular and molecular level.