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Note positive staining in nonneoplastic colonic mucosa (A) but negative staining in nonneoplastic small intestinal mucosa (B; original magnifications X40 [A] and X100 [B]).
2) to compare the sensitivity of negative staining of Heine with modified negative staining method.
In terms of diagnosis of malignancy, the combination of cytology and positive staining for CEA as well as negative staining for CAL reached a diagnosis in 29 of 30 metastatic adenocarcinoma cases, with 97% sensitivity, 100% specificity and 98% accuracy.
Knob-tike protrusions found on the surfaces of SVs, apparent after negative staining, have previously been regarded as the vacuolar ATP-dependent proton pump (V-ATPase) (Wilkens et al.
3] positive sites on the long arm, probably indicating AgNOR, while with the fluorochrome DAPI these regions showed negative staining and were therefore rich in GC bases.
Histochemical stains showed negative staining for PAS with diastase predigestion (dPAS), and in a few epithelial cases, mucicarmine stained focal areas.
Kedersha turned to negative staining, a microscopy method as simple as it is messy.
NanoImaging offers a variety of services, from visual inspection and statistical characterization to sophisticated 3D reconstruction and antibody/labeling experiments, using TEM procedures that range from conventional negative staining to advanced cryo techniques with automated image acquisition and analysis.
Myoepithelial (MEP) Markers MEP Markers Cellular MEP Stromal Vessels Localization Cells Myofibroblasts p63 Nuclear +++ - - Calponin Cytoplasm +++ + ++ SMMHC Cytoplasm +++ + ++ SMA Cytoplasm +++ +++ +++ Basal-type Cytoplasm +++ - - CKs (a) and membrane Abbreviations: CKs, cytokeratins;SMA, smooth muscle actin; SMMHC, smooth muscle myosin heavy chain; +, weak, positive staining; ++, medium, positive staining; +++, strong, positive staining; -, negative staining.
Immunohistochemistry revealed positive staining with S-100 protein, Vimentin, smooth muscle actin (SMA) and negative staining for Estrogen and Progesterone Receptors.
Table 1: Criteria for Immunohistochemical Analysis of Percentage of Cells (6) Expressing the Marker Negative Staining <1% tumour cells show positive staining Weak Positive Staining 1-10% of tumour cells show weak positive staining Strong Positive Staining >10% of tumour cells show positive staining Expression of CEA in Comparison with Other Markers VIM+ VIM- ER+ ER- PR+ PR- CEA+ 1 16 1 16 2 15 CEA- 10 3 9 4 6 7 Expression of Vimentin in Comparison with Other Markers CEA+ CEA- ER+ ER- PR+ PR- VIM+ 1 10 10 1 6 5 VIM- 16 3 0 19 1 18 Table 2: Association of Tumour Grade with CEA Expression Grade Positive Negative Total Well Differentiated 3 11 14 Moderately Differentiated 11 1 12 Poorly Differentiated 3 1 4 Total 17 13 30 Chi-Square Test p value 0.