A coated grid with sample adsorbed to the surface is floated on a drop of negative stain
for 0.5-2 min, excess stain wicked away with a piece of filter paper, air dried for 1-3 min, and examined by electron microscopy (Figure 4D).
The larger diameter can be explained by flattening of the vesicles upon drying down in the negative stain
(16) Negative stain
electron microscopy is a useful method for addressing questions concerning size distribution of the liposome, and it is a reliable technique that is simple to perform and requires only limited specialized equipment.
The morphologies of purified virus particles, as revealed by EM after heavy metal shadowing and staining with different negative stains
, are shown in Fig 1.
Caption: Figure 7: Bladder tumor with negative stain
for GATA 3 in neoplastic cells.
EM and biochemical/biophysical and cell-based assays.
Grids with adherent axoplasm are immediately transferred to a 10-[mu]l droplet of motility buffer (3) and then passaged through three 10-[mu]l droplets of 1mg/ml bacitracin and three droplets of 2% aqueous uranyl acetate for 1 min each as a negative stain
Thin section and negative stain
electron microscopy (EM) examination of viruses grown in cultured cells have been instrumental in determining an etiologic agent in numerous disease outbreaks caused by previously unknown viruses.
electron microscopy was performed by using cell culture supernatant.
(104) Positive immunostains of P501S and NKX3.1 together with a negative stain
of GATA3 support prostate carcinoma, and diagnosis of urothelial carcinoma would be supported by GATA3 (Figure 7, D) positivity and negative stain
of P501S and NKX3.1.
Positive NM23 stain (score 3) (Figure 1.) was observed in 30 (53.3%) and negative stained
in 26 (46.4%) cases of adjacent gastric mucosa (Figure 2.).
Table 3: Negative Stains
Immunohistochemical Representative Cells or Tissue marker Calretinin Mesothelium CD1a Langerhans Cells CD4 T Lymphocytes CD15 Histiocytes, Neutrophils, Adenocarcinoma CD23 B Lymphocytes, Follicular Dendritic Cells CD34 Blood Vessels, Hematopoietic Stem Cells CD45 Lymphoid Tissue CD117 Gastrointestinal Stromal Tumor, Mast Cells CDX2 Transcription factor of intestinal organogenesis, found in Colorectal and Gastric Adenocarcinoma Chromogranin Neuroendocrine Cells CK7 Adenocarcinoma CK20 Adenocarcinoma HMB45 Melanoma PAX-5 B Lymphocytes, Neuroendocrine Tumor, Wilms Tumor, Breast and Urothelial Carcinoma PSA Prostate PSAP Prostate Synaptophysin Neuroendocrine Cells TTF-1 Lung, Thyroid Summary of immunohistochemical markers that stained negative in this case.