negative stain


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neg·a·tive stain

stain forming an opaque or colored background against which the object to be demonstrated appears as a translucent or colorless area; in electron microscopy, an electron opaque material, such as phosphotungstic acid or sodium phosphotungstate, is used to give detail to surface structure.

neg·a·tive stain

(neg'ă-tiv stān)
Stain forming an opaque or colored background against which the object to be demonstrated appears as a translucent or colorless area; in electron microscopy, an electron opaque material, such as phosphotungstic acid or sodium phosphotungstate, is used to give detail as to surface structure.
References in periodicals archive ?
The negative stain methylamine tungstate tolerates collection of the 140 images typically needed for a tomography series, yielding reconstructions of SV surfaces with exquisite detail.
16) Negative stain electron microscopy is a useful method for addressing questions concerning size distribution of the liposome, and it is a reliable technique that is simple to perform and requires only limited specialized equipment.
119) The differential diagnosis of YST from spermatocytic tumor could be further confirmed by immunoreactivity of GPC3 and AFP in YST and negative stain in spermatocytic tumor.
This finding was quickly corroborated by negative stain EM, IHC assay, serologic testing, and molecular assays.
A coated grid with sample adsorbed to the surface is floated on a drop of negative stain for 0.
Rotavirus infections were diagnosed by negative stain electron microscopy.
A negative stain preparation may be made and a result could be obtained within 5 minutes of the specimen's arrival in the EM laboratory.
The ambiguous group included those with other combinations of stains, including negative stains for all markers.
Table 3: Negative Stains Immunohistochemical Representative Cells or Tissue marker Calretinin Mesothelium CD1a Langerhans Cells CD4 T Lymphocytes CD15 Histiocytes, Neutrophils, Adenocarcinoma CD23 B Lymphocytes, Follicular Dendritic Cells CD34 Blood Vessels, Hematopoietic Stem Cells CD45 Lymphoid Tissue CD117 Gastrointestinal Stromal Tumor, Mast Cells CDX2 Transcription factor of intestinal organogenesis, found in Colorectal and Gastric Adenocarcinoma Chromogranin Neuroendocrine Cells CK7 Adenocarcinoma CK20 Adenocarcinoma HMB45 Melanoma PAX-5 B Lymphocytes, Neuroendocrine Tumor, Wilms Tumor, Breast and Urothelial Carcinoma PSA Prostate PSAP Prostate Synaptophysin Neuroendocrine Cells TTF-1 Lung, Thyroid Summary of immunohistochemical markers that stained negative in this case.
With negative stains, the report reads: "No herpes simplex isolated.
Despite negative stains and cultures, the identification of DNA from M.