The results indicated that the native proteins
from PSE-like meat have less functionalities including the lower thermal gelling ability and less effectively trapping water.
A large positive z-score indicates a wide gap between the energy of the native protein
and the mean decoy energy.
A marked quenching of the intrinsic fluorescence compared to the native protein
has also been reported for BAA at pH 2.25 .
The ranking of SNPs on the basis of PolyPhen-2 scores enables us to assess the potential-quantitative effect of SNPs on native protein
Random amino acid sequences were generated on the basis of native proteins
by the RandSeq program (http://au.expasy.org/tools/randseq.htm) to serve as a control in estimating protein similarity.
The activity of isolated trypsins on native protein
substrates was evaluated using two model proteins (BSA and myoglobin).
Native-PAGE was analyzed for native protein
color with the method of SDS-PAGE, without initial denaturation and SDS ddition.
Mass spectroscopy was used to calculate the mass of native protein
eluted from gel filtration (gpMucB).
In Western blotting% recombinant GRA5 was detected as a band of about 18 kDa, which was about the same size as the native protein
Sequence comparison showed that most of Cry2 proteins in the GenBank present more than 50 amino acid differences throughout the protein, not only with the native protein
, but also between Cry2 proteins reported.
Examples of topics covered include the methods for homologous recombination, the use of recombinases in gene activation/inactivation and transgenesis, immunolabeling of embryos, imaging cellular and molecular dynamics in live embryos using fluorescent proteins, imaging of neuromuscular junctions, imaging pupal wing morphogenesis, microscopic analysis of the adult Drosophila retina using semithin plastic sections, extraction and immunoblotting of proteins from embryos, mass production of embryos and chromatographic purification of native protein
complexes, and flow cytometric analysis of Drosophila cells.
SDI's Genomic Antibodies(TM) have demonstrated the ability to produce specific antibodies to native protein
. The process is capable of simultaneously delivering large numbers of antibody reagents, each with a unique specificity, facilitating large-scale biomarker identification, drug development, and academic research.