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The nRNA fluorescence of the RNAse-treated nuclei was significantly less than the values seen for the positive control (DAPI?
Therefore, replicate measurements of nRNA fluorescence from each tank for each treatment were pooled.
Plots of nRNA and DNA fluorescence showed that always-fed larvae had a distinct group of aggregated S-phase nuclei that joined the G0/G1 and G2/M phases, and S-phase nuclei were fewer and dispersed for unfed larvae (Fig.
There was no significant difference in overall nRNA fluorescence between the always-fed and unfed treatments (ANOVA, [F.
There was a loss of DNA when larvae were frozen and thawed, but that process did not affect nRNA measurements.
We developed a protocol for staining nRNA in muscle cell nuclei of larvae of Walleye Pollock for use in flow cytometry, and we showed that the inclusion of an nRNA covariate in a model resulted in more accurate measurement of physiological condition than did cell-cycle analysis alone.
This result also indicates that our nRNA staining protocol worked as intended.
An nRNA covariate improved larval condition measurements.
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- NRMI 2
- NRMI 3