In this study, our finding showed that the aged ApoE-/- mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aortic segments mounted in myographs
. In contrast, TXL improved endothelium-dependent vasodilatation in ApoE-/- mice, indicating its protective effect on the vascular ECs, which proved the results of other clinic trials, and that mechanism probably correlated with elevating eNOS activity and increasing NO synthesis.
After sacrifice, aorta was immediately isolated and mounted onto myograph
. (A) Aortic contractile response to phenylephrine (PE).
Maximal voluntary isometric force was measured using a myograph
which consisted of a load cell (100 N), a peak-and-hold meter and a visual feedback monitor.
Mesenteric arteries were dissected free from surrounding tissue and mounted on a small vessel wire myograph
for measurement of isometric tension (n=5/gender/group).
Family Mortuary of Eugene is in charge of arrangements.
The heart was dissected free, after removal of chest plate, and connected by means of a hook to a "B" myograph
. Impulses from the myograph
were fed into an E and M physiograph and recorded on a moving paper.
Uterine horn contractions were monitored on a myograph
(Model F-60, Narco Bio-Systems) connected to a pen recorder (Narcotrace 40 physiograph, Narco Bio-Systems) set at a sensitivity of 50 mV/cm.
The aortic rings were mounted onto two stainless steel wires and installed on a wire myograph
(Model 620M, Danish Myo Technology, Aarhus, Denmark), filled with 5 ml PSS buffer aerated with 95% [O.sub.2] and 5% C[O.sub.2] at 37[degrees]C, which linked to transducers to recorded data.
Their segments (~2mm long) were placed in a Mulvany-Halpern isometric myograph
(620 M, DMT, Denmark) and incubated in modified Krebs-Henseleit solution (KHS, mmol/l: 119 NaCl, 4.7 KCl, 1.17 MgS[O.sub.4], 25 NaHC[O.sub.3], 1.18 K[H.SUB.2]P[O.SUB.4], 2.5 CaCl2, and 2 g/l glucose; 37[degrees]C; bubbled with 95% O2 and 5% C[O.sub.2]).
Individual arterial ring segments having 2 mm length were mounted in a four channel small vessel wire myograph
(model 510M Danish Myotech, Aarus, Denmark).
Vasodilatation was measured in mesenteric arteries with a wire myograph
. Nitrites were measured by fluorescence with 2,3-diaminonaphthalene and [H.sub.2][O.sub.2] was measured with carbon microsensors.