In this study, our finding showed that the aged ApoE-/- mice had lower endothelium-dependent vasorelaxation elicited by acetylcholine in aortic segments mounted in
myographs. In contrast, TXL improved endothelium-dependent vasodilatation in ApoE-/- mice, indicating its protective effect on the vascular ECs, which proved the results of other clinic trials,[13] and that mechanism probably correlated with elevating eNOS activity and increasing NO synthesis.[14]
After sacrifice, aorta was immediately isolated and mounted onto
myograph. (A) Aortic contractile response to phenylephrine (PE).
Maximal voluntary isometric force was measured using a
myograph which consisted of a load cell (100 N), a peak-and-hold meter and a visual feedback monitor.
Mesenteric arteries were dissected free from surrounding tissue and mounted on a small vessel wire
myograph for measurement of isometric tension (n=5/gender/group).
Myograph Family Mortuary of Eugene is in charge of arrangements.
The heart was dissected free, after removal of chest plate, and connected by means of a hook to a "B"
myograph. Impulses from the
myograph were fed into an E and M physiograph and recorded on a moving paper.
Uterine horn contractions were monitored on a
myograph (Model F-60, Narco Bio-Systems) connected to a pen recorder (Narcotrace 40 physiograph, Narco Bio-Systems) set at a sensitivity of 50 mV/cm.
The aortic rings were mounted onto two stainless steel wires and installed on a wire
myograph (Model 620M, Danish Myo Technology, Aarhus, Denmark), filled with 5 ml PSS buffer aerated with 95% [O.sub.2] and 5% C[O.sub.2] at 37[degrees]C, which linked to transducers to recorded data.
Their segments (~2mm long) were placed in a Mulvany-Halpern isometric
myograph (620 M, DMT, Denmark) and incubated in modified Krebs-Henseleit solution (KHS, mmol/l: 119 NaCl, 4.7 KCl, 1.17 MgS[O.sub.4], 25 NaHC[O.sub.3], 1.18 K[H.SUB.2]P[O.SUB.4], 2.5 CaCl2, and 2 g/l glucose; 37[degrees]C; bubbled with 95% O2 and 5% C[O.sub.2]).
Individual arterial ring segments having 2 mm length were mounted in a four channel small vessel wire
myograph (model 510M Danish Myotech, Aarus, Denmark).
Vasodilatation was measured in mesenteric arteries with a wire
myograph. Nitrites were measured by fluorescence with 2,3-diaminonaphthalene and [H.sub.2][O.sub.2] was measured with carbon microsensors.