Corticosteroids are also alleged to cause glucocorticoid receptor-mediated cross linkage of actin-filament network21 and an expression of protein myocilin
in trabecular meshwork impeding aqueous outflow.22 Patients with diabetic mellitus are more prone to have higher IOP and increased prevalence of POAG and ocular hypertension.
Currently known genes associated with POAG include Myocilin
(MYOC), Atonal BHLH transcription Factor 7 (ATOH7), Transmembrane and Coiled-Coil Domains 1 (TMCO1), SIX Homeobox 1/SIX Homeobox 6 (SIX1/SIX6), Growth Arrest Specific 7 (GAS7), (Abu-Amero et al.
Two candidate genes associated more broadly with other subtypes of glaucoma, myocilin
(MYOC), and lysyl oxidase homolog 1 (LOXL1) have shown limited association with PDS/PG.
A reduction of myocilin
mRNA was observed, suggesting the feasibility of translating this technology to patients with MYOC mutations.
The factors associated with pathogenesis of glaucoma include high intraocular pressure (IOP), increased oxidative stress, aging, glutamate neurotoxicity, and susceptibility genes such as optineurin and myocilin
In six affected and three asymptomatic members, gene sequencing revealed a mutation c.C1456T in exon 3 of myocilin
OLFM3 is an olfatomedin-related protein that interacts with myocilin
(Torrado et al., 2002), which is a major cause of glaucoma and may play a role in cytoskeletal function (Stone et al., 1997).
Sodium 4-Phenylbutyrate Acts as a Chemical Chaperone on Misfolded Myocilin
to Rescue Cells from Endoplasmic Reticulum Stress and Apoptosis.
A study was undertaken on 30 patients (25 male and 5 females; mean age at onset of disease being 59.23 years) of primary open angle glaucoma (POAG) and similar no (sixteen male and 14 females; mean age at onset of disease being 54.10 years) primary angle closure glaucoma (PACG) for (i) analyzing mutations in myocilin
(MYOC) gene, (ii) inheritance pattern of MYOC gene, (iii) comparing the frequency of MYOC gene in northern and southern population, and (iv) detect genotype and phenotype correlation based on mutations.
The suitability of DNA extracted from tissue sections was confirmed by the amplification of the cellular gene Myocilin
, using primers capable of amplifying conserved 200 bp of coding sequence (primer sequence in the Table).
Papers includes the age and racial variations, risk factors for open-angle glaucoma, risk factors in various isolated populations, mechanisms of intraocular pressure elevation, genetics of glaucoma (including susceptibility and myocilin
mutations), molecular and cellular responses in the eye to glaucoma, ocular tissue and psycho-physiological responses, current and successful models, and therapeutic modalities.