The method is based on development of profiles of mycolic acids
, which vary from one species to another.
Valle, Synthesis and biological aspects of mycolic acids
: an important target against Mycobacterium tuberculosis, Scientific World J, 8, 720 (2008).
The slide was then covered with carbolfuchsin (1%) and heated to enable the dye to bind the cell wall mycolic acids
of the bacterium.
Considering the fact that primary target of INH involves inhibition of mycolic acids
synthesis [5, 8-11], understanding other classes of lipid compositional changes in MTB upon INH treatment may further help in identification of potential novel targets.
The unique characteristic of mycolic acids
in Dietzia may indicate a novel fatty biosynthesis .
Activated isoniazid interferes with the biosynthesis of essential mycolic acids
through inhibition of nicotinamide adenine dinucleotide hydrogen (NADH) dependent enoylacyl carrier protein reductase, which is encoded by inhA promoter.
Gal-3 has also been demonstrated to bind lipooligosaccharides from Neisseria gonorrhoeae  and mycolic acids
from mycobacteria .
Furthermore, these observations could also be due to the fact that mycobacteria have unique cell envelope known as mycomembrane comprising mycolic acids
which are known to govern pathogenicity of mycobacteria and that iron deprivation may possibly affect mycolic acid
synthesis as well.
Among the fats, sugars and proteins in this coat are fatty molecules called mycolic acids
. The Help Stop TB project will use the computing power donated by World Community Grid members to simulate the behavior and chemical properties of mycolic acids
to better understand how they protect the TB bacteria.
Saturated fatty acids with no mycolic acids
were detected using gas chromatograpy.
Lepromatous leprosy patients produce antibodies that recognise nonbilayer lipid arrangements containing mycolic acids
. Mem Inst Oswaldo Cruz, 2012; 107: 95-103.
These mycolic acids
, along with free lipids, create a hydrophobic effect.