Ellingson and coauthors (2005) cultured milk on HEYM slants with mycobactin
J and amphotericin B, nalidixic acid, and vancomycin.
paratuberculosis (MAP) by inoculation of the AFB positive faecal samples on Middlebrook 7H10 media supplemented with mycobactin
J (1 [micro]g/ml) (OIE Terrestrial Manual 2008) after following appropriate decontamination procedure by Merkal and Richards (1972).
Ratledge, "Equivalence of mycobactins
from Mycobacterium senegalense, Mycobacterium farcinogenes and Mycobacterium fortuitum" Journal of General Microbiology, vol.
The salicylate-derived mycobactin
siderophores of Mycobacterium tuberculosis are essential for growth in macrophages.
MAP organisms were grown on Middlebrook 7H10 agar enriched with 0.1% glycerol v/v and 10% oleic acid dextrose catalase (OADC) with additional supplementation of Mycobactin
J (2mg/l) while E.
However, MAP is dependent on mycobactin for its growth, whereas M.
Microbiologic analyses included culture for mycobacteria (liquid media: BACTEC 460TB or MGIT [Becton, Dickinson and Company, Cockeysville, MD] and solid media produced inhouse, all media without supplementation of mycobactin) from at least 21 specimens (blood, urine, sputum, biopsy, feces) over a 3-year period.
Three different siderophores mycobactins
(Francis et al., 1949), ferrichrome (Neilands, 1952) and coprogen (Hesseltine et al., 1952) were isolated as growth factors during 1949 to 1952.
tuberculosis sends out small molecules called mycobactins
to rip iron out of the human proteins that carry the metal, he says.