(2007) carried out a thorough investigation on 1001 Indian cows and buffaloes of various breeds; the FXI deficient cattle were detected with 0.6% mutant frequency
. Patel and Patel (2014) reviewed that the FXI deficiency gene had been extensively studied in Indian Holstein cattle.
At the low dose, brain showed a 7-fold increase in mutant frequency (p < 0.0001), liver showed a 3-fold increase (p = 0.04), and bone marrow was unaffected (p = 0.13).
These pooled samples had significantly higher mutant frequencies than lower doses and controls (Figure 3A; 16-fold, p = 0.0003), and the average mutant frequency (46.3 x [10.sup.-5]) was 4-fold higher than that seen in sperm of adult males exposed to 100 mg/kg/day N-ethyl-N-nitrosourea (ENU) (O'Brien et al.
Transgenic rodent assay for quantifying male germ cell mutant frequency. J Vis Exp 90:e51576, doi: 10.3791/51576.
Mutant frequency determination was performed as described in OECD guideline for tk locus mutation assay .
Viability and mutant frequency were analyzed by calculating related indices, such as relative suspension growth (RSG), plate efficiency (PE0, PE2), relative survival rate (RS), and mutant frequency (MF) using standard methods .
Aardema, "The spectral karyotype of L5178Y TK+/- mouse lymphoma cells clone 3.72C and factors affecting mutant frequency at the thymidine kinase (tk) locus in the microtitre mouse lymphoma assay," Environmental and Molecular Mutagenesis, vol.
Efficiency for unique identification of mutants in arrays from pool coordinates alone is theoretically achieved by choosing n and m on the basis of expected mutant frequency
and empirically possible pooling ratio [11, 12].
The mutant frequency (Mt) in the cloning assay in 10 high-exposure workers was significantly higher (p < 0.05) than the Mf in 11 controls (Mf [+ or -] SD = 17.63 + 5.05 x [10.sup.-6] vs.
Our results at this point consistently indicate that exposure to BD at about 1-3 ppm is associated with an increase in HPRT mutant frequency.
The mutant frequency significatively increased during the fourth week after the exposure: 13.5 x [10.sup.-5] in the exposed group versus 6.9 x [10.sup.-5] in the control group.
Mutant frequency and mutation spectrum determinations.