multiple reaction monitoring

A multistage/mass spectrometry (MS/MS) experiment that uses two sequential stages of independent mass analysis. In the product ion MS/MS scan, a precursor ion is selected by mass with the first mass analyser, and the fragment ions formed by collision-induced dissociation are measured with a scan of the second mass analyser and recorded in a mass spectrum.
In an analogy to selected ion monitoring, if both mass analysers in an MS/MS instrument are set on a specific mass, the signal represents the precursor-to-product ion transition for a specific ion pair and is termed reaction monitoring. If several different precursor–product ion pairs are monitored, as is the norm, the analysis is termed multiple reaction monitoring.

References in periodicals archive ?

The global multiple reaction monitoring assay market is expected to grow moderately due to increasing research and development activities in the biologics field.

Now Available: Global Multiple Reaction Monitoring Assay Market Forecast and Growth 2016-2026

1] Nonstandard abbreviations: MRM3, multiple reaction monitoring with multistage fragmentation; UHPLC-MS/ MS, ultra- high performance LC-MS/MS; Mr, relative molecular mass.

Interference from 3-O-methyldopa with ultra-high performance LC-MS/MS measurements of plasma metanephrines: chromatographic separation remains important

MRM Proteomics, is recognized as a pioneer and leading company in the development of mass spec based methods for protein quantification using multiple reaction monitoring," said Steve Fischer, Agilent marketing manager, Metabolomics and Proteomics.

United States : Agilent Technologies, MRM Proteomics Sign Comarketing Agreement to Further Quantitative Proteomics Solutions

Traditionally, triple-quadrupole mass spectrometers have been used for these targeted, multiplexed analyses due to the excellent selectivity, large dynamic range, high sensitivity and low limits of quantitation obtained with multiple reaction monitoring (MRM) in triple-quadrupole MS instruments.

Bruker Introduces MS Workstation 7 Software for New Chemical Analysis Gas Chromatography Triple-Quadrupole Mass Spectrometers

Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins.

Quantification of serum IgG subclasses by use of subclass-specific tryptic peptides and liquid chromatography-tandem mass spectrometry

In these applications, where peptides are measured by multiple reaction monitoring (MRM), the improved column-to-column reproducibility delivered by the cHiPLC-nanoflex helps in obtaining consistent data from numerous samples.

Docking station enhances nanoLC for proteomics

Multiple Reaction Monitoring (MRM) allows for drastic reduction or elimination of matrix interferences that restrict the accuracy or detection limits of other techniques.

TRADE NEWS: Agilent Technologies Introduces Two Factory-Configured Analyzers to Detect Trace Levels of Broad Range of Targeted Pesticides

Multiple reaction monitoring (MRM) was performed with a 5-ms dwell time per SRM channel and an interchannel delay time of 5 ms, resulting in a data acquisition cycle of 0.

High-throughput assay of 9 lysosomal enzymes for newborn screening

The 7000B MS/MS achieves ultra-fast multiple reaction monitoring (MRM) speed of 500 per second without any "cross talk" between consecutive transitions.

TRADE NEWS: Agilent Technologies' New Triple Quadrupole GC/MS Offers Greater Precision, Selectivity and Sensitivity

NYSE:A) today announced a collaboration to create the Human Multiple Reaction Monitoring (MRM) Atlas, a comprehensive resource designed to enable scientists to perform quantitative analysis of all human proteins.

TRADE NEWS: Institute for Systems Biology, Agilent Technologies to Build MRM Atlas for Quantification of All Human Proteins

In particular, widely used triple-quadrupole selected reaction monitoring or multiple reaction monitoring measurement platforms allow targeted multiplexed tandem MS detection of hundreds of peptides during an LC separation, providing limits of detection and quantification several orders of magnitude lower than presently feasible with discovery-based platforms.

Mass spectrometry in biomarker applications: from untargeted discovery to targeted verification, and implications for platform convergence and clinical application

We were able to take the same Liquid Tissue processed samples used in discovery and measure the relative expression of candidate biomarkers with recent advances in Multiple Reaction Monitoring.

Expression Pathology Aids Research in Identifying Biomarkers of Lung Cancer Metastasis and Survival