multiple reaction monitoring

A multistage/mass spectrometry (MS/MS) experiment that uses two sequential stages of independent mass analysis. In the product ion MS/MS scan, a precursor ion is selected by mass with the first mass analyser, and the fragment ions formed by collision-induced dissociation are measured with a scan of the second mass analyser and recorded in a mass spectrum.
In an analogy to selected ion monitoring, if both mass analysers in an MS/MS instrument are set on a specific mass, the signal represents the precursor-to-product ion transition for a specific ion pair and is termed reaction monitoring. If several different precursor–product ion pairs are monitored, as is the norm, the analysis is termed multiple reaction monitoring.

References in periodicals archive ?

The global multiple reaction monitoring assay market is expected to grow moderately due to increasing research and development activities in the biologics field.

Now Available: Global Multiple Reaction Monitoring Assay Market Forecast and Growth 2016-2026

Ossola et al., "High sensitivity detection of plasma proteins by multiple reaction monitoring of N-glycosites," Molecular and Cellular Proteomics, vol.

Current status and advances in quantitative proteomic mass spectrometry

Siu, "Absolute quantification of potential cancer markers in clinical tissue homogenates using multiple reaction monitoring on a hybrid triple quadrupole/linear ion trap tandem mass spectrometer" Analytical Chemistry, vol.

Protein target quantification decision tree

The LCMS-8030 features multiple reaction monitoring (MRM) transitions, enabling data acquisition with as many as 500 different channels per second.

Pharmatek expands Mfg. capabilities: Shimadzu offers enhanced mass spectrometer

Correct identification of the BoNT product peptides depends on both a retention time match, with respect to standards, and on a chemical-specific fragmentation (a precursor to product ion multiple reaction monitoring [MRM] transition) monitored by tandem MS.

Botulinum neurotoxin detection and differentiation by mass spectrometry

We used multiple reaction monitoring (MRM) [1] to measure the steroid hormones cortisol, cortisone, progesterone, testosterone, androstenedione, 11-deoxycortisol (11DOC), 17[alpha]-hydroxyprogesterone (17OHP), and corticosterone.

Use of micro-HPLC-MS/MS method to assess diurnal effects on steroid hormones

Seven different tryptic peptides are monitored using multiple reaction monitoring (amino acid residues, peptide number): SV SEIQLMHNLGK (1-13, P1), LM HNLGK (7-13, P2), HLNSMER (14-20, P3), LQDVHNFVALGA PLAPR (28-44, P4), FVALGAPL APR (34-44, P5), VALGAPLAPR (35-44, P6), and ADVNVLTK (73-80, P7).

Mass spectrometric immunoassay raises doubt for the existence of parathyroid hormone fragment 7-84