multiple reaction monitoring

A multistage/mass spectrometry (MS/MS) experiment that uses two sequential stages of independent mass analysis. In the product ion MS/MS scan, a precursor ion is selected by mass with the first mass analyser, and the fragment ions formed by collision-induced dissociation are measured with a scan of the second mass analyser and recorded in a mass spectrum.
In an analogy to selected ion monitoring, if both mass analysers in an MS/MS instrument are set on a specific mass, the signal represents the precursor-to-product ion transition for a specific ion pair and is termed reaction monitoring. If several different precursor–product ion pairs are monitored, as is the norm, the analysis is termed multiple reaction monitoring.

References in periodicals archive ?

The global multiple reaction monitoring assay market is expected to grow moderately due to increasing research and development activities in the biologics field.

Now Available: Global Multiple Reaction Monitoring Assay Market Forecast and Growth 2016-2026

1] Nonstandard abbreviations: MRM3, multiple reaction monitoring with multistage fragmentation; UHPLC-MS/ MS, ultra- high performance LC-MS/MS; Mr, relative molecular mass.

Interference from 3-O-methyldopa with ultra-high performance LC-MS/MS measurements of plasma metanephrines: chromatographic separation remains important

MRM Proteomics, is recognized as a pioneer and leading company in the development of mass spec based methods for protein quantification using multiple reaction monitoring," said Steve Fischer, Agilent marketing manager, Metabolomics and Proteomics.

United States : Agilent Technologies, MRM Proteomics Sign Comarketing Agreement to Further Quantitative Proteomics Solutions

Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins.

Quantification of serum IgG subclasses by use of subclass-specific tryptic peptides and liquid chromatography-tandem mass spectrometry

In these applications, where peptides are measured by multiple reaction monitoring (MRM), the improved column-to-column reproducibility delivered by the cHiPLC-nanoflex helps in obtaining consistent data from numerous samples.

Docking station enhances nanoLC for proteomics

Multiple reaction monitoring (MRM) was performed with a 5-ms dwell time per SRM channel and an interchannel delay time of 5 ms, resulting in a data acquisition cycle of 0.

High-throughput assay of 9 lysosomal enzymes for newborn screening

In particular, widely used triple-quadrupole selected reaction monitoring or multiple reaction monitoring measurement platforms allow targeted multiplexed tandem MS detection of hundreds of peptides during an LC separation, providing limits of detection and quantification several orders of magnitude lower than presently feasible with discovery-based platforms.

Mass spectrometry in biomarker applications: from untargeted discovery to targeted verification, and implications for platform convergence and clinical application