multilocus enzyme electrophoresis


Also found in: Acronyms.

mul·ti·lo·cus en·zyme e·lec·tro·pho·re·sis

(MLEE) (mŭl'tē-lō'kŭs en'zīm ĕ-lek'trō-fŏr-ē'sis)
A nonamplified strain-typing method. Proteins are isolated from the strain of interest and separated in a gel. A probe is used to detect a specific protein.
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References in periodicals archive ?
meningitidis infection has historically been addressed by using multilocus enzyme electrophoresis (MEE) (4,5).
Despite the availability of a number of meningococcal typing strategies (including pulsed-field gel electrophoresis, multilocus enzyme electrophoresis, and 16s rRNA typing), to date, no portable method is broadly accepted for identifying subvariants below the level of clonal complex.
avium) were likely responsible for the infections based on the identity of patient and hot tub mycobacterial isolates by either restriction fragment length polymorphism analysis (24) or multilocus enzyme electrophoresis (2,3).
The most variable, lap with 20 alleles, was expected because it is a highly variable locus when analyzed with multilocus enzyme electrophoresis (25).
Comparison of multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for molecular subtyping of Cryptococcus neoformans.
meningitidis by multilocus enzyme electrophoresis (MLEE), random amplification of polymorphic DNA (RAPD), and MLST; population genetic analyses confirmed that the population structure of these bacteria is clonal (28).
When MAC isolates were examined by multilocus enzyme electrophoresis (MEE), however, the hot tub and patient isolates had different MEE patterns.
Multilocus enzyme electrophoresis (MLEE) has been the reference method for global epidemiology of Neisseria meningitidis.
Molecular subtyping, primarily by multilocus enzyme electrophoresis (MEE) and ribotyping, has identified substantial genetic diversity within the Corynebacterium diphtheriae species, leading to the identification of a unique clonal group that emerged in Russia in 1990 at the beginning of the current diphtheria epidemic (1).