Network and frequency profiles of the 18 rice frogs haplotypes based on the mtDNA
D-Loop region (Fig.
There are numerous reports of brain tumors that have proven to be associated with the presence of mtDNA
control region was amplified by polymerase chain reaction (PCR) using the following primers: mitM1 (5'-GG AGACTAACTCCGCCATCA-3'; nucleotide positions 15379-15398 of pig mtDNA
sequence FJ237003) and mitM2 (5'-TT TTGGGGATGCTTAGACTCA-3'; positions 15995-16016).
The right ulna originally disinterred with the remains eventually yielded an mtDNA
sequence not matching that of the other elements, confirming the suspicion of commingling within the NMCP casket.
To identify mutations in mtDNA
, DNA sequencing was performed.
In this work, we explored the mtDNA
T16189C polymorphism in populations of subjects with or without MetS diagnosed based on NCEP/ATP III criteria.
Preparation of standard dilutions: A 68 bp unique sequence of mtDNA
was amplified using mitochondrial forward and mitochondrial reverse primers.
About 90% of cellular ROS is produced by the mitochondria [14, 15] and high levels of ROS are associated with mtDNA
Clearly, significant mtDNA
hypomethylation was observed in cells following senescence (Figure 3(e)).
Sequencing, Genotyping, and Rebuilding the mtDNA
For the analysis of the mtDNA
D-loop region, fifteen unrelated, Nili-Ravi type buffaloes were selected from the irrigated agriculture belt of the Nili and Ravi Bar regions of the Punjab province in Pakistan.
An analysis of 280 chromosomally normal blastocysts showed that 15 (5.4%) contained unusually high levels of mitochondrial DNA (mtDNA
) and the remaining blastocysts had normal or low mtDNA