Counterstaining for nuclei was performed using the Vectashield mounting medium
for staining with DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride, Vector Laboratories Inc., Burlingame, CA, USA).
The sessile ciliates were mounted in Gray & Wess between a slide and a coverslip, since this mounting medium
was very satisfactory for this group of parasites.
Pitfalls to be avoided include improper centering of the medium droplet on the slide, using too little medium to accommodate the coverslip, and failure to rinse reactive macerants from the specimen prior to transfer to the mounting medium
." Thus, for the novice, getting specimens properly prepared for identification with the keys in this volume is a substantial task.
The brain tissues were placed in a cryostat and 30[micro]m sections were cut, free-floating in PBS, mounted on to glass slides and finally embedded with a fluorescence mounting medium
Arterioles were mounted on microslides using either the Prolong Antifade mounting medium
from Molecular Probes, Inc., or the Aqua Poly Mount mounting medium
from Polysciences, Inc., both of which are designed for fluorescence microscopy.
Labeled larvae were mounted under coverslips in either DPX mountant (Electron Microscopy Sciences) after ethanol dehydration or in a glycerol mounting medium
containing 5% n-propyl gallate (Giloh and Sadat, 1982).
If it is filled with mounting medium
or some other clear substance, the structure appears clear or translucent in transmitted light, or nearly invisible in reflected light.
We have selected silver flake (or tin flake for preparing sulfides since sulfides reacts with silver) as a mounting medium
since they have good electrical conductivity, bleed the charge and help to conduct away the heat generated from the electron beam sample interaction and do not add an excessive number of peaks to the x-ray spectrum at analysis time.
Tissue in excess of what was necessary for diagnostic purposes was obtained <15 min after removal from the patient, embedded in cryostat mounting medium
(TISSUE-Tek O.C.T.; Sakura Finetek U.S.A.), and frozen in liquid nitrogen.
All the dry-mounted glands (prior to the addition of the mounting medium
) resemble typical schizogenous glands with intact epithelial cells.
Avoid destroying evidence by shotblasting Preserve cavity linings by filling with mounting medium
prior to polishing Avoid contamination from polishing medium/ water Prepare plane through unexposed portion of cavity
There's no need to embed the objects in a mounting medium
(such as synthetic resin) or to press them in plasticine.