solanacearum which showed positive reactions for potassium hydroxide solubility test, catalase test, starch hydrolysis test, motility test
and casein hydrolysis test and showed negative reaction for gram staining.
tarda: Edwardsiella tarda; FAO: Food and Agricultural Organization; FISH: Fluorescence in Situ Hybridization; g/l: Gram per liter; [H.sub.2][O.sub.2]: Hydrogen Peroxide; [H.sub.2]S: Hydrogen Sulfide; HPCE: Higher Performance Capillary Electrophoresis; LAMP: Loop-Mediated Isothermal Amplification; LFDP: Lake Fisheries Development Working Paper; ml: Milliliter; PCR: Polymerase Chain Reaction; SIM: Sulfur Indole and Motility Test
Media; TSA: Tryptic Soya Agar; TSIA: Triple Sugar Iron Agar; USA: United State of America; V/V: Volume by Volume; WHO: World Health Organization; XLD: Xylose Lysine Deoxycholate
Bacterial isolates were observed for colony morphology including shape of bacterial colonies, colour of bacterial colonies, texture of colonies and studied microscopically by gram's staining, endospore staining and motility test
Biochemical test Klebsiella Escherichia pneumonia coli Catalase test (+)ve (+) ve Oxidase test (-)ve (-) ve simmon citrate test (+)ve (-) ve Urease test (+)ve Yellow/Yellow with gas production(-)ve TSI (+) ve (+) ve Indol test (-)ve (+) ve Motility test
(-)ve (+)ve Methyl red test (-)ve (+) ve Vogas-proskaure test (+) ve (-) ve Gram stain (-)ve (-) ve Cell morphology On Macconkey agar Smooth, glassy and bacterial colonies translucent, rosy have a viscous/mucoid pink on MacConkey appearance with the appearance of metallic sheen when growing on Eosin methylene blue.
In the light of history, clinical examiation and omasal motility test
, omasal impaction was diagnosed.
A motility test
, with and without NA, was performed on motility agar made of 0.4% (w/v) agar (Oxoid), 10g/L peptone (Oxoid), 5g/L Nacl (Merck), 3 g/L Beef extract (Oxoid), and 0.05 g/L of 2, 3, 5 triphenyl tetrazolium chloride (Sigma).
For the preparation of motility test
medium, 3 g of the motility agar was added in 100 ml distilled water.
The biochemical tests of API 20 Strep (bioMerieux, Marcy l'Etoile, France) and motility test
at 30[degrees]C were used to characterize the isolate at the species level.
'The sperm motility test
enables us to objectively measure how well sperm from each male can swim in a solution at body temperature, possibly mimicking the environment in the hen's reproductive tract.'
Lactic Acid Bacteria isolates preliminary screen out by Grams reaction, catalase activity and motility test
Biochemical test Klebsiella Escherichia pneumonia coli Catalase test + ve + ve Oxidase test - ve - ve simmon citrate test + ve - ve Urease test + ve Yellow/Yellow with gas production(-)ve TSI + ve + ve Indol test - ve + ve Motility test
- ve + ve Methyl red test - ve + ve Vogas-proskaure test + ve - ve Gram stain - ve - ve Cell morphology On Macconkey agar Smooth, glassy and bacterial colonies translucent, rosy have a viscous/ pink on MacConkey mucoid appearance with the appearance of metallic sheen when growing on Eosin methylene blue.