In order to better understand the results obtained in the second experiment, the experiment 3 (n=619) aimed to assess fertilization and monospermy rates after IVF of low quality oocytes.
05), the rate of monospermy in the group pre incubated for 1.
5h improved monospermy in low quality oocytes, even in comparison to high quality oocytes fertilized with not pre-incubated semen.
Allele patterns for CHMs were consistent with monospermy in 25 cases (81%) and dispermy in 6 cases (19%) (Figure 1).
Monospermy was the most frequently observed mechanism for CHM (81%), whereas dispermy was always observed for PHM, consistent with previous studies.
Results of Microsatellite Genotyping for 102 Cases Nonmolar N 54 Monospermy 0 Dispermy 0 Additional abnormalities Trisomy 7 (n = 1) Trisomy 8 and trisomy 18 (n = 1) Trisomy 13 (n = 1) Trisomy 16 (n = 7) Trisomy 21 (n = 1) Androgenetic/biparental mosaicism (n = 1) Cross-contamination Fetal (n = 2) Maternal (n = 2) Average No.
In pigs, high sperm concentration and POEC increase oocyte penetrability and decrease monospermy rates when sperm pre-culture with POEC is limited to 2 hr; the presence of POEC during IVF increased the rate of monospermy.
In porcine, the oviduct-specific glycoprotein (OVGP1) increases the rate of monospermy in in vitro fertilized oocytes through induction of zona hardening and interaction with spermatozoa (42).
39 (d) Percentage of oocytes BHB concentration (mM) N * with monospermy
0 30 45.
Monospermy is ensured after the fast polyspermy block by the collaboration of the two strategies--suppression of the acrosome reaction of the supernumerary sperm and blockage of sperm entry at the plasma membrane.
due to the egg membrane hyperpolarization at fertilization, ensures physiological monospermy in eggs of the crab Maia squinado.