The inoculum medium was prepared with a solution of 20 g [L.sup.1] xylose, 3 g [L.sup.1] yeast extract, 5 g [L.sup.1] peptone, 1 g [L.sup.1] magnesium sulfate (Mg[SO.sub.4]), 5 g [L.sup.1] monobasic
potassium phosphate (KH2PO4) and 3 g [L.sup.1] ammonium sulfate [[([NH.sub.4]).sub.2][SO.sub.4]].
Laying hens respond differently to high dietary levels of phosphorus in monobasic
and dibasic calcium phosphate.
Deionized water was produced in-house, and acetonitrile, methanol, and potassium phosphate monobasic
powder of HPLC grade were purchased from Sigma Aldrich.
After digestion, the samples were centrifuged at 200 xg for 10 min, and the pellet was resuspended in an erythrocyte lysis solution containing 154 mM ammonium chloride, 5.7 mM monobasic
potassium phosphate, and 0.1 mM EDTA pH7.3 for 10 min.
The following drugs were used to execute the experimental protocols: Croton oil, hexadecyltrimethylammonium bromide (HTAB), tetramethylbenzidine (TMB), dexamethasone, rutin, gallic acid, ascorbic acid, chlorogenic acid, rosmarinic acid, solanine, DPPH, ammonium phosphate monobasic
(all from Sigma, St.
Mushroom tyrosinase, chlorogenic acid, epicatechin, schisandrin, and sodium phosphate monobasic
were purchased from Sigma-Aldrich (St.
Histolojik inceleme icin %10 dogal tamponlu formol cozeltisinde (Sodium phosphate monobasic
: 4,0 g, Sodium phosphate dibasic: 6,5 g, formaldehit %37: 100,0 mL, distile su 900,0 mL, pH 6,8) fiske edilen dokular %70-%98 araliginda uc seri etil alkol, standart uc seri ksilol ve uc seri parafin solusyonlarindan gecirilerek gomme ve bloklama islemleri yapilmistir.
Cryptophthalma Cobos (1982) is monobasic
. Muona et al.
After the incubation period, the doses of 0, 140, 280, 560 and 1280 mg [kg.sup.-1] of [P.sub.2][O.sub.5] were added in the form of monobasic
ammonium phosphate and homogenized in the entire volume of the substrate.
Ammonium formate, silica, sodium phosphate monobasic
, sodium citrate tribasic dehydrate, sodium acetate, taurocholic acid sodium salt hydrate, N-acetylgalactosamine and D-saccharic acid 1,4 lactone monohydrate were purchased from SigmaAldrich.
It was recommended that among the priority hazardous substances in Estonia, the substances that need more attention and periodic monitoring should include monobasic
phenols (sum of p-, m-, o-cresols; 3,4-, 3,5-, 2,3-, 2,6-dimethyl phenols, and phenol), dibasic phenols (sum of resorcinol, 2,5-dimethyl resorcinol, and 5-methyl resorcinol), some heavy metals [4,5] and, for geological reasons, barium .
Samples were processed through 10% buffered formalin fixative (100 ml formalin, 900 ml distilled water, 4 g monobasic
NaH2PO4, 6.5g dibasic Na2HPO4) for 48 h.