molecular beacon


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molecular beacon

a dual labelled OLIGONUCLEOTIDE PROBE, with a fluorescent reporter group at one end and a fluorescence quencher group at the other, that reports the presence of target nucleic acid molecules in solution by fluorescing. In the absence of a HYBRIDIZATION target, the probe forms an internal HAIRPIN structure that brings the reporter and quencher groups into close proximity, resulting in the quenching of fluorescence. In the presence of a hybridization target, the probe molecule unfolds and hybridizes to the target. Reporter and quencher are now physically separated and the reporter dye can fluoresce on stimulation, indicative of hybrid formation. Such a probe is useful in situations where it is not possible or desirable to isolate probe-target hybrids from an excess of the probe.
References in periodicals archive ?
The researchers compared the sensitivity and accuracy of molecular beacon and TaqMan[R] probe PCR assays with the conventional USDA microbiological procedure using artificially contaminated RTE meats.
Since the molecular beacon probe has the advantages of strong specificity, short hybridization time, and easy operation [19-22], it is expected to be applied in the miRNA rapid detection and analysis.
Ling, "Fluorometric determination of Simian virus 40 based on strand displacement amplification and triplex DNA using a molecular beacon probe with a guanine-rich fragment of the stem region," Microchimica Acta, vol.
Currently, the World Health Organisation (WHO) has endorsed molecular beacon technology for the direct detection of MTB by using ultra-sensitive polymerase chain reaction (PCR) technology.
This decision is a major independent validation of Miacom's proprietary molecular beacon technology being successfully applied in the hemoFISH assays.
Detection is by a fluorescently-labeled molecular beacon. Fluorescence is released as the fluorophore and quencher are separated when the beacon stem is opened.
For the signal system, TaqMan probe was used instead of molecular beacon (Table 1).
The ANSR system uses an innovative isothermal DNA amplification process and fluorescent molecular beacon technology for detection of the pathogen target.
The molecular beacon sensing probe based on FRET was usually applied in DNA detection, in which the donor and acceptor were connected by a typical DNA strand with a stem-loop beacon structure.
MOLECULAR BEACON. Steven Xu Wu *, Julia Xiaojun Zhao
Rapid detection and quantitation of bluetongue virus (BTV) using a molecular beacon fluorescent probe assay.
The sensitivity and accuracy of molecular beacon and TaqMan[R] probe PCR assays were compared with the conventional USDA microbiological procedure using artificially contaminated RTE meats.
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