The researchers compared the sensitivity and accuracy of molecular beacon
and TaqMan[R] probe PCR assays with the conventional USDA microbiological procedure using artificially contaminated RTE meats.
Since the molecular beacon
probe has the advantages of strong specificity, short hybridization time, and easy operation [19-22], it is expected to be applied in the miRNA rapid detection and analysis.
Ling, "Fluorometric determination of Simian virus 40 based on strand displacement amplification and triplex DNA using a molecular beacon
probe with a guanine-rich fragment of the stem region," Microchimica Acta, vol.
Currently, the World Health Organisation (WHO) has endorsed molecular beacon
technology for the direct detection of MTB by using ultra-sensitive polymerase chain reaction (PCR) technology.
This decision is a major independent validation of Miacom's proprietary molecular beacon
technology being successfully applied in the hemoFISH assays.
Detection is by a fluorescently-labeled molecular beacon
. Fluorescence is released as the fluorophore and quencher are separated when the beacon stem is opened.
For the signal system, TaqMan probe was used instead of molecular beacon
The ANSR system uses an innovative isothermal DNA amplification process and fluorescent molecular beacon
technology for detection of the pathogen target.
The molecular beacon
sensing probe based on FRET was usually applied in DNA detection, in which the donor and acceptor were connected by a typical DNA strand with a stem-loop beacon structure.
. Steven Xu Wu *, Julia Xiaojun Zhao
Rapid detection and quantitation of bluetongue virus (BTV) using a molecular beacon
fluorescent probe assay.
The sensitivity and accuracy of molecular beacon
and TaqMan[R] probe PCR assays were compared with the conventional USDA microbiological procedure using artificially contaminated RTE meats.