modified acid-fast stain

modified acid-fast stain

a stain for coccidia (Cryptosporidium, Cyclospora, Isospora) in which the decolorizer is a highly acid (1-3% sulfuric acid) and is less likely to remove too much dye.

mod·i·fied acid-fast stain

(mod'i-fīd as'id-fast stān)
An acid-fast staining procedure using highly diluted acid as a decolorizer, suitable for detection of coccidia, Cryptosporidium, Cyclospora, and Isospora, which bind function stains less firmly than mycobacteria.
References in periodicals archive ?
The two initial cases, reported in the June 28 issue of EPI Update, were diagnosed by a laboratorian at SHL who identified possible Cyclospora oocysts on microscopic examination of fresh stool and confirmed this diagnosis using a modified acid-fast stain technique.
A modified acid-fast stain is performed to identify parasites such as Cryptosporidium and Isospora, causing chronic diarrhoea in immunocompromised patients.
Gram stain (Figure, C) and a modified acid-fast stain (Figure, D) revealed gram-positive, partially acid-fast filamentous organisms branching at right angles within the eosinophilic material consistent with Nocardia organisms.
The bug was considered a rare and exotic pathogen until the mid-1980s when a modified acid-fast stain was employed to look for Cryptosporidium in stool specimens, especially from people with impaired immune systems who suffered from HIV disease.[6] A much larger organism was frequently associated with enteritis and was variously described in subsequent reports as a large Cryptosporidium, a fungal spore, a member of the Blastocystis species, a cyanobacterium-like body (CLB, or blue-green algae), and coccidian-like.[6-9] These organisms probably were Cyclospora and have become associated with travel or residence in developing countries.[10]
They were stained with Kinyoun's carbol fuchsin modified acid-fast stain and examined by oil immersion microscopy.
All state laboratories began routine testing with a modified acid-fast stain for C.
However, with the modified acid-fast stain, CLB may stain deep mottled red or pink; some resist staining and appear as glassy, membranous cysts.
Use of modified acid-fast stains, such as Kinyoun stain, also improves visualization [12].
CDC recommends reconfirmation of positive test results (by using direct fluorescent antibody testing or modified acid-fast stained smears) obtained with ImmunoCard STAT![R] rapid assays from all recalled lots.
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