All reads in each sample were mapped back to the transcriptome using Bowtie 2  (default parameters used, but end to end, allowing two bases of mispairing
and multiple hits [less than or equal to] 20) and then used to estimate expression values for each transcript by RSEM .
The majority of the mutations (67 of 99) are A x T [right arrow] G x C transitions, which could result from either incoming dCTP mispairing
with template A or incoming dGTP mispairing
with template T.
Examination of the mutational spectra in these mutant tissues reveals a distinct preponderance of G:C transversions, an observation consistent with the fact that polycyclic aromatic compounds bind primarily to the N2 position of guanine, causing replicative mispairing
that converts G:C base pairs to mutant base pairs.
They usually generate mispairing
, base pair substitutions and small deletions and insertions in genomes.
These DNA fragments are produced during PCR by slipped strand mispairing
mechanism and also were seen in the results of other studies (30), (31).
Characteristic genomic fluidity (e.g., facilitated by transformation, slipped-strand mispairing
, or 2-component regulatory pathways) enables N.
Although the 6-thienyl group of s efficiently prevents the mispairing
with the natural pyrimidine bases, y has no such functional group to exclude mispairing
with the natural purine bases.
 Levinson, G., and Gutman, G.A., 1987, "Slipped-strand mispairing
: A major mechanism for DNA sequence evolution," Mol.
riparius chromosomes have been eliminated as a result of mispairing
Halivni notes that he had been "looking for quite a while for an opportunity to write about the mispairing
of sin and Holocaust.
: A major mechanism for DNA sequence evolution, Molecular Biology and Evolution (1987) 4(3):203-221.