Generally anticancer agents intermingle with the DNA by four bonding modes such as interaction with minor groove
or major groove, surface binding and intercalation.
While the cytotoxins used in the majority of advanced programs in the field prevent tubulin polymerization during cell division, Synthon's differentiating linker-drug technology - applying valine-citrulline-seco-DUocarmycin- hydroxyBenzamide-Azaindole (vc-seco-DUBA) - is based on synthetic duocarmycin analogs, which bind to the minor groove
of DNA and subsequently cause irreversible alkylation of DNA.
ACKR1 was genotyped using a 5'-nuclease assay (NA) with TaqMan minor groove
binding (MGB) probes.
After the addition of a couple (two to three) of CPPs, the major groove is filled, and peptides preferentially bind to the minor groove
of the siRNA, as seen in Figures 5A2 and 5B2.
Docking studies also suggested the minor groove
binding of the Schiff bases with DNA.
The contract provides MGB Biopharma with access to additional intellectual property owned by University of Strathclyde, relating to DNA Minor Groove
This has been an ongoing challenge of molecular biologists, with few approaches emerging, such as peptide nucleic acids (PNA), triplex forming oligonucleotides (TFO), minor groove
binding polyamides, and engineered proteins.
MGB Biopharma's anti-infectives are based on its minor groove
binder (MGB) technology.
Addition of a minor groove
binder (MGB) to the probe sequence at the 3' end of the probe permits it to anneal at a higher melting temperature (Tm), allowing the use of shorter more specific probes (2,18).
The minor groove
binder (MGB) probe technology overcomes some of these obstacles.
Initially isolated from the marine animal Ecteinascidia turbinate, it binds selectively with the minor groove
of DNA, and drives cells to apoptosis by interfering with DNA repair and transcription factors.
This assay amplifies and detects a conserved region upstream of the Rotbart amplicon by using primers containing degenerate and modified bases and a minor groove
binder (MGB)-conjugated hybridization probe.