Complete feather degradation by these isolates was determined by using 20ml minimal medium
(0.03% K2HPO4, 0.04% KH2PO4, 0.05% NaCl, 0.01% MgCl2) with 0.5% (w/v) native feathers as the sole source of carbon.
coli strains were grown at 37[degrees]C with aeration at an agitation rate of 180 rpm in LB medium (for overnight growth) or M9 minimal medium
(containing 6.4 g/L [Na.sub.2]HP[O.sub.4]*7[H.sub.2]O, 1.5g/L K[H.sub.2]P[O.sub.4], 0.25 g/L NaCl, and 0.5 g/L N[H.sub.4]Cl) supplemented with 2 mM MgS[O.sub.4], 0.1 mM Ca[Cl.sub.2], 0.1 mM casamino acids, and 1% glycerol as the sole carbon source (for growth during the analysis).
Notwithstanding the lower growth rate in minimal medium
plus xylose, the growth inhibition haloes were evident for both A, baumannii and K, pneumoniae in the presence of either tetracycline or chloramphenicol.
The bacteria were screened by growing and acid-producing ability on minimal medium
with a low carbohydrate content (Table 1).
Fungicide solutions were prepared by making their stocks in water and then required amount of this pesticide solution (calculated according to their recommended dose) was added to the minimal medium
. The fungicide solutions were prepared fresh, when required.
Standard serial dilution method was used for isolation of xylano lytic fungi on Reese's Minimal Medium
(RMM) with 1% beechwood xylan SRL Pvt.
After the applied treatments, the colonies were transferred to minimal medium
(Burk's Nitrogen Free agar medium) and complete medium i.e.
Using a protein derived from human blood called Inter-alpha inhibitor, the researchers grew human pluripotent stem cells in a minimal medium
without the need for costly and time-consuming biological substrates.
To investigate possible symbiotic relationships, the microbes were co-cultured individually or in pair-wise combinations with G12 in a minimal medium
that did not favor growth when species were grown individually.
coli on agar plates with and without MSG, and in minimal medium
with various amounts of MSG.
The minimal medium
containing different concentrations of carbon sources and nitrogen sources were initially used to optimize the effect of carbon and nitrogen source on growth of the bacteria and prodigiosin production at 37[degrees]C and the pH was maintained at 6.8.
Strain SPG was grown in a 500 mL Erlenmeyer flask containing 200 mL minimal medium
, 0.5 mM indole, and 10 mM sodium succinate, and the flask was incubated at 30[degrees]C under shaking (200 rpm).