Representative photographs are presented (left; magnification, x200), and the relative number of migratory cells
(right) were counted.
Images of migratory cells
from the scratched boundary were observed and acquired with light microscope (magnification 10 x 10).
Having overcome cell adhesion, migratory cells
must penetrate the basement membrane.
These cells then send chemical signals through the blood to recruit more of the migratory cells
, which continue to enter the sphere.
Finally, the nonmigratory cells were scraped off and washed gently in PBS, the migratory cells
on the bottom surface of the transwell membrane were fixed in cold methanol for 30 min and underwent crystal violet staining, then photographed under a digital microscope (Olympus BX51; Olympus, Tokyo, Japan).
Biologists at the California Institute of Technology (Caltech) led by an Indian origin researcher have now found that neural-crest stem cells-multipotent, migratory cells
unique to vertebrates that give rise to many structures in the body such as facial bones and smooth muscle-also play a key role in building olfactory sensory neurons in the nose.