microwave antigen retrieval

microwave antigen retrieval

Heat treatment of a tissue sample to increase the retrieval (“unmasking”) of an antigen and enhance the tissue’s immunoreactivity.
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Effects of tissue fixatives on antigen preservation for immunohistochemistry: a comparative study of microwave antigen retrieval on Lillie fixative and neutral buffered formalin.
Well-controlled immunohistochemical stains for CK and EMA (DakoCytomation, Carpinteria, Calif) were performed on all cases with sufficient histologic material according to established protocols using the diaminobenzidine detection with microwave antigen retrieval in a citrate buffer.
Microwave antigen retrieval in 1 mmol/L EDTA buffer solution at pH 8.
A conventional avidin-biotin complex method was used with microwave antigen retrieval.
Although the exact mechanism concerning microwave antigen retrieval is not clear, it is possible that this technique restores antigenicity by unfolding of keratin or an interacting protein.

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