microwave antigen retrieval

microwave antigen retrieval

Heat treatment of a tissue sample to increase the retrieval (“unmasking”) of an antigen and enhance the tissue’s immunoreactivity.
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References in periodicals archive ?
Effects of tissue fixatives on antigen preservation for immunohistochemistry: a comparative study of microwave antigen retrieval on Lillie fixative and neutral buffered formalin.
Commercially available primary monoclonal antibody against CD20 (clone UCHT1, Dako) was used to stain 3.0 [micro]m sections of liver biopsies after deparaffinization by routine microwave antigen retrieval and horseradish peroxidase (diaminobenzidine) kit (DAKO, Carpinteria, CA), following manufacturer's instructions.
One or more of the following antibodies were used after microwave antigen retrieval: Cytokeratin AE1/AE3 (CK AE1/AE3, Dako, Germany, prediluted); CD31 (Dako, Germany, 20:1000); CD34 (Dako, Germany, 10:1000); Factor VIII (Dako, Germany, 20:1000); CD68 (Dako, Germany,10:1000); and Epithelial Membrane Antigen (EMA, Dako, Germany, 5:1000).
Well-controlled immunohistochemical stains for CK and EMA (DakoCytomation, Carpinteria, Calif) were performed on all cases with sufficient histologic material according to established protocols using the diaminobenzidine detection with microwave antigen retrieval in a citrate buffer.
A conventional avidin-biotin complex method was used with microwave antigen retrieval. Appropriate positive and negative control slides were stained in parallel.

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