However, the presence of microvesicular steatosis
on core liver biopsy eventually confirmed the diagnosis of AFLP.
Brunt et al., "Presence and significance of microvesicular steatosis
in nonalcoholic fatty liver disease," Journal of Hepatology, vol.
The liver biopsy results showed mild macrovesicular and microvesicular steatosis
with mild portal and lobular inflammation.
Liver biopsy showed severe macro- and microvesicular steatosis
with associated severe fibrosis and portal ductular reaction (Figure 1).
These data included donor age, gender, body mass index (BMI), biopsy findings (hydrops, sinusoidal dilatation, pigment accumulation, inflammatory infiltration, parenchymal focal necrosis, microvesicular steatosis
, and macrovesicular steatosis), graft type, volumetric analysis of the liver calculated by multi-slice computerized tomography (CT) [total liver volume (TLV), functional liver volume (FLV), graft volume (GV), remnant liver volume (RLV), percentage of remnant liver volume to total liver (RLV%), remnant liver to donor body weight ratio (RLBWR)], graft weight after hepatectomy (GW), peri-operative use of blood transfusion, fresh frozen plasma (FFP), amounts of crystalloid and colloid solutions, operation time, as well as intraoperative and postoperative complications.
2D) and microvesicular steatosis
was observed in the E5 group (Fig.
Likewise, NAFLD grading showed that, of the WT-HFD mice, three had stage 2 (predominantly microvesicular steatosis
with mild lobular inflammation), three had stage 3 (mixed steatosis with lobular inflammation and ballooning degeneration), and one had stage 4 (mixed steatosis with lobular inflammation and ballooning degeneration and macrovesicular steatosis, associated with portal and lobular inflammation, Councilman's bodies, ballooning degeneration, Mallory's hyaline, and fibrosis) NAFLD.
Arrows indicated appearance of prominent microvesicular steatosis
. Values were presented as the mean [+ or -] SD.
As shown in Figures 2(a) and 2(b), mice fed the HFD developed severe macrovesicular and microvesicular steatosis
without apparent inflammatory cell infiltration and fibrosis, while there appeared to be an attenuated state of steatosis with notable reduction in the number of vacuolar areas in both Curc-mPEG454 treatment groups.
Cases in which histopathology sections were showing mainly microvesicular steatosis
and those cases where aetiology of steatosis could not be confirmed were excluded from the study.
, as seen with tetracycline and valproate, results from acute impairment of [beta] oxidation of fatty acids in mitochondria.
According to these criteria, macrovesicular steatosis is quantified based on the percentage of involved hepatocytes (0 = absent; 1 < 33%; 2 = 33-66%; 3 > 66%), and its zonal distribution and the presence of microvesicular steatosis
are noted; hepatocellular ballooning is evaluated for zonal location, and the estimate of its severity (mild, marked) is based on the numbers of hepatocytes showing this abnormality .