In this way, it would destabilize microtubule
assembly, cargo and samurai proteins dynamicity (such as dynein, cofactor dynactin, kinesin, and samurai protein katanin), vesicle transport.
For chromosomes and nuclei, eggs labeled for either microtubules
or for centrosomes were then counterstained with DAPI stock solution (DAPI 5 [micro]g/mL, 2-mercapt-ethylamine hydrochloride 10 mM, Tris 10 mM, EDTA-2Na 100 mM, NaCl 100 mM) diluted to 0.
Further examination of the microtubule
arrays at telophase showed that the microtubules
were arranged at the equatorial plate in the CK and B treatments (Fig.
Given that curcumin interacts with microtubules
to suppress their dynamic instability (Banerjee et al.
Among our observations, particularly striking was the accumulation of centralspindlin on antiparallel bundles of overlapping astral microtubules
that accompany the ingressing cleavage furrow.
At that point, we have structures that want to do work -- the kinesin-powered microtubules
-- and something they want to do work on -- the spheres," Paxton said.
A thick fiber of the doublet microtubules
was extruded from the anterior end of the fibrous sheath and formed a loop at the midpiece (Fig.
Citation: "CLASP2 interacts with pl20-catenin and governs microtubule
dynamics at adherens junctions"; M.
All of these routes to spindle formation appeared to be dependent on Augmin - a protein complex responsible for amplifying the number of microtubules
in the cell.
Based on their observations, the team derived a simple mathematical model describing the relationship between the size and shape of the spindle apparatus and the density and amount of microtubules
Quadilobing in certain liverworts is preceded by pre-meiotic bands of microtubules
marking the future cytokinetic planes before the nucleus enters prophase I (Fig.
The binding of kinetochores to microtubules
likely involves cooperative interactions between different copies of the kinetochore components.