Abbreviations: bl--basal lamina; gc--generative cell; h--hemocyte; hc--hepatic cell; is--intercellular space; jo--junction occlusion; m--mitochondria; mv--microvilli; n-- nuclei; rer--rough endoplasmic reticulum; arrow in (A): spherocrystal; arrow in (B): spaces observed in the cytoplasm resulting from the removal of spherocrystals during microtomy
samples were prepared by boiling the 0.02-[m.sup.2] cubes in glycerin for 3 hours to soften and were sectioned in the radial, tangential, and axial dimensions using a sliding microtome.
(11.) Vercammen, Y., De Mondt, R., Van Luppen, J., Vangaever, F., and Van Vaeck, L., "Ultra-Low-Angle Microtomy
and Static Secondary Ion Mass Spectrometry for Molecular Depth Profiling of UV-Curable Acrylate Multilayers at the Nanoscale," Anal.
Although embedment with subsequent polishing or microtomy
may be considered labor-intensive, these methods permit precise, reproducible sample preparation.
The fiber sample was embedded in epoxy, cured, and sectioned by microtomy
. Inspection of cross sections by optical microscopy, shown in Figure 9, revealed the presence of a skin layer around the microfibers.
The illustrated chapters include fixation and processing, microtomy
, frozen sections, hematoxylin and eosin, Gram stain, myobacteria, Heliobacter pylori, spirochetes, fungi, trichrome stains, reticulin, elastin stains, basement membranes, mucin stains, amyloid, and immunohistochemistry.
(31) invented the in-plane microtomy
to study the migration of ultraviolet light absorber (UVA) and hindered amine light stabilizer (HALS) between coating layers.
One of the key problems with the HistoGel-only method is that the pellet formed by this method may be poorly visible in the paraffin block, thus precluding sectioning at the correct level during microtomy
. After trials of several dyes and marker materials (including banana peel, as previously described (11)), we found that a simple and practical marker was a short (3-5 mm) segment of dark, synthetic surgical suture (2-0 Ethilon nylon suture, Johnson and Johnson, New Brunswick, New Jersey) [Figure 2, A through D].
in addition, a sample preparation trimmer for serial block face sem and ultra microtomy
, as well as a tissue infiltration unit are required.
Another technical problem that affects the accuracy of the measurements is the tissue undergoes dramatic dimensional changes during processing and microtomy
. Sometimes, correction factors should be determined for the Retraction--which occurs 3-D, and compression--that happens in the direction of the cut of the microtome knife (Weibel, 1979).
It is also possible that the stress relaxation occurring around the cavities during microtomy
of the ultra-thin foils increase their size compared to that existing in the bulk material.
The powder was embedded in an epoxy resin (polaron 612) before microtomy
. The magnetic properties of the samples were measured using a superconducting quantum interference device (MPMS magnetometer) at room temperature.