Oligonucleotide probes at 10 [micro]mol/L in phosphate-buffered saline (pH 7.4; Sigma-Aldrich) supplemented with 1 mmol/L EDTA were diluted 2-fold by the addition of Array-it Microspotting
Solution Plus (Telechem International).
Many of the benchtop arrayers used by researchers to manufacture customized arrays in-house rely on mechanical microspotting
. DNA deposition results from direct surface contact between the printing substrate and the delivery mechanism, such as an array of pins or capillaries.
A contact printing robot (PixSys 5500; Cartesian Technologies) with a stealth microspotting
pin (Model SMP3; TeleChem International) was used to print the protein chips on the aldehyde-activated slides.