The results showed that the initial abortive development of sterile
microspores occurred at meiosis I.
cytokinesis produces two diploid
microspores. Even though the first
One of the most popular methods for production of haploids is through anther and
microspore culture on the artificial culture medium.
These irregularities culminated in the formation of
microspores 2n (Figure 2E), as well as the micronuclei produced
microspores with reduced amount of genetic material, being called unbalanced
microspores.
The buds containing more percentage of anther with early to late uni-nucleate stage
microspores were selected and stored moistened in plastic bags for cold pretreatment at 4C for different time intervals (0 24 48 72 96 hrs) in dark (Fig.
At this stage, the wall surrounding the four
microspore cells dissolve and the
microspores in the pollen sac are released.
Another hypothesis is that orbicules participate actively in sporodenn formation by representing a transport system of sporopollenin between the tapetum and the developing
microspores. This idea is based on observations of connections between orbicules and pollen sexines, mainly in grasses, or observations of a close contact between
microspores and orbicules in other species.
scitamineum was as follows: teliospores germinate, germ tube, promycelium, basidiospore, and
microspore, which was similar to the observation of mycelial growth process of Sporisorium reilianum in maize seedling by Zhang et al.
Micronuclei have been observed in 4.19% of the analyzed
microspores (Figure 3G).
microsporangia: Receptacle in which
microspores (male spores) are developed.
Theoretically, the pores or the imperfections on the chemically attacked surfaces were as a result of three reasons: Firstly, due to the remaining
microspores resulting from unfinished densification; secondly, the existence of [beta]-TCP phase as the decomposition product which dissolved much faster than the pure HA phase; thirdly, the intrinsic chemical stability of the ceramics [19].
In this study, we developed two optimal media for in vitro culture of
microspores and germination of mature pollen in Petunia hybrida.