The results showed that the initial abortive development of sterile microspores
occurred at meiosis I.
cytokinesis produces two diploid microspores
. Even though the first
One of the most popular methods for production of haploids is through anther and microspore
culture on the artificial culture medium.
These irregularities culminated in the formation of microspores
2n (Figure 2E), as well as the micronuclei produced microspores
with reduced amount of genetic material, being called unbalanced microspores
The buds containing more percentage of anther with early to late uni-nucleate stage microspores
were selected and stored moistened in plastic bags for cold pretreatment at 4C for different time intervals (0 24 48 72 96 hrs) in dark (Fig.
At this stage, the wall surrounding the four microspore
cells dissolve and the microspores
in the pollen sac are released.
Another hypothesis is that orbicules participate actively in sporodenn formation by representing a transport system of sporopollenin between the tapetum and the developing microspores
. This idea is based on observations of connections between orbicules and pollen sexines, mainly in grasses, or observations of a close contact between microspores
and orbicules in other species.
scitamineum was as follows: teliospores germinate, germ tube, promycelium, basidiospore, and microspore
, which was similar to the observation of mycelial growth process of Sporisorium reilianum in maize seedling by Zhang et al.
Micronuclei have been observed in 4.19% of the analyzed microspores
microsporangia: Receptacle in which microspores
(male spores) are developed.
Theoretically, the pores or the imperfections on the chemically attacked surfaces were as a result of three reasons: Firstly, due to the remaining microspores
resulting from unfinished densification; secondly, the existence of [beta]-TCP phase as the decomposition product which dissolved much faster than the pure HA phase; thirdly, the intrinsic chemical stability of the ceramics .
In this study, we developed two optimal media for in vitro culture of microspores
and germination of mature pollen in Petunia hybrida.