microspectrophotometry


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mi·cro·spec·tro·pho·tom·e·try

(mī'krō-spek'trō-fō-tom' ĕ-trē),
A technique for characterizing and quantitating nucleoproteins in single cells or cell organelles by their natural absorption spectra (ultraviolet) or after binding stoichiometrically in selective cytochemical staining reactions, as in the Feulgen stain for DNA.
See also: cytophotometry.

mi·cro·spec·tro·pho·tom·e·try

(mī'krō-spek'trŏ-fō-tom'ĕ-trē)
A technique for characterizing and quantitating nucleoproteins in single cells or cell organelles by their natural absorption spectra (ultraviolet) or after binding stoichiometrically in selective cytochemical staining reactions, as in the Feulgen stain for DNA.

microspectrophotometry

(mī″krō-spĕk″trō-fō-tŏm′ĕ-trē)
Method for the histochemical study of substances present in cells, such as nucleic acid, based on absorption in the ultraviolet spectrum. This method permits quantitative and qualitative studies of certain cellular components.
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References in periodicals archive ?
Make and model determination of hit-and-run vehicles by reflectance microspectrophotometry, Journal of Forensic Sciences (1986) 31:464-470.
Especially suited to the critical demands of microspectrophotometry, the new lenses transmit between 230 nm and 2,100 nm.
Abbreviations: MSP, microspectrophotometry; PIA, phylogenetically informed annotation; TEM, transmission electron microscopy.
Comparison of minute smears of lipstick by microspectrophotometry and scanning electron microscopy/energy-dispersive spectroscopy, Journal of Forensic Sciences (1991) 36:366-375.
DNA microspectrophotometry of shoot apical meristem cell populations in Ceratopteris thalictroides (Filicales).
Transmission and reflectance microspectrophotometry of inks, Journal of Forensic Sciences (1988) 33:1171-1184.
The low genome size variation observed in this study more closely agreed with the other flow cytometric studies than with the microspectrophotometry study.
Cronin and Jinks (2001) reviewed structural and spectral aspects of crustacean visual ontogeny and predicted the presence of two receptor classes in some crustacean larvae despite the fact that microspectrophotometry has found only a single visual pigment.
This module is intended to familiarize the trainee with the use of ultraviolet/visible light microspectrophotometry as a qualitative, quantitative and objective method of color analysis.
Nuclear DNA content in plants was previously determined by feulgen microspectrophotometry of root tip or shoot tip mitotic cells (Bennett and Smith, 1976).
Further study using intracellular recording or microspectrophotometry is needed to clarify all the spectral cell types.