Biochemical analysis of microsomal enzyme
Klaassen, "Induction of hepatic glutathione S-transferases in male mice by prototypes of various classes of microsomal enzyme
inducers," Toxicological Sciences, vol.
Liver cytochrome P450 is a microsomal enzyme
present only in liver.
In the adrenal cortex, this is processed into either glucocorticoids and androgens in zona fasciculate and zona reticularis (which contain the microsomal enzyme
cytochrome P450c17[alpha]-hydroxylase: P450c17 and 17,20 lyase) or into mineralocorticoids in zona glomerulosa (which does not contain P450c17 and 17,20 lyase, but contains aldosterone synthase, CYP11B2).
AST is a microsomal enzyme
that is found in large amounts in the liver and with destructive liver tissue, is released in the blood in large amounts .
During phase I metabolism, drug molecules are transformed through oxidation or reduction of hydrolysis reactions by the action of a family of enzymes called the Cytochrome P450 (CYP) or microsomal enzyme
DGAT is a microsomal enzyme
expressed in the liver and rate-limited enzyme in the process of TG synthesis, catalyzes the final step in TG synthesis by converting diacylglycerol and fatty acylcoenzyme A into TC (Farese et a).
For assay after incubation of chemicals with the rat liver microsomal enzyme
system, a sample from the incubation mixture was subjected to the assay as described above.
Results did demonstrate that TBT neither directly inhibits the ATAT enzyme responsible for the fatty acid esterification of testosterone, nor does it suppress levels of the microsomal enzyme
, as determined though activity assays following in vivo exposure to TBT.
Increase in rat liver UDP-glucuronosyltransferase mRNA by microsomal enzyme
inducers that enhance thyroid hormone glucuronidation.
The reaction mixture contained 0.1 M potassium phosphate buffer (pH 7.5), 3 mM NADH, 5 mM potassium ferriciyanide and microsomal enzyme
in a final volume of 1.0 ml.
The hepatoprotective effect may be mediated through combination of multiple mechanisms, such as reported anti-oxidant, free radical scavenging, anti-inflammatory, calcium channel blocking and/or microsomal enzyme