microinjection


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microinjection

(mī′krō-ĭn-jĕk′shən)
n.
Injection of minute amounts of a substance into a microscopic structure, such as a single cell.

mi′cro·in·ject′ (-jĕkt′) v.

microinjection

A method of introducing DNA into a cell by direct injection into the nucleus, using a microcapillary pipette.

microinjection

a method for introducing new DNA into a CELL by injecting it directly into the NUCLEUS or CYTOPLASM using a thin glass needle. The technique has been used to inject cloned genes into the nucleus of an egg cell in vitro, to generate a TRANSGENIC ORGANISM.
References in periodicals archive ?
Activity of fCas9 was proven in animals; for instance, mice bearing mutation on the Bcr locus were generated by microinjection of fCas9 and gRNAs into fertilized eggs (22).
A novel system consisting in a mold with inserts and heating elements to control the temperature of the surface of a mold for microinjection molding was developed by De Santis and coworkers [11, 12].
Lim et al., "Localized intradermal microinjection of tranexamic acid for treatment of melasma in Asian patients: A preliminary clinical trial," Dermatologic Surgery, vol.
As shown in Figures 5(a) and 5(b), microinjection of baclofen into the NTS induced an elevation in BP in varying degrees in different groups.
(4) RF/DVC+ Va group: the microinjection of 1microgram remifentanil for 1 [micro]L into the DVC was carried out on the vagotomized rats, followed by the HIR procedure.
The microinjection of saline (100-150 nL, n=10) into the CnF did not affect [DELTA]MAP (before: 72.1[+ or -]4.59 mm Hg, after: 76.4 3.3 mm Hg) or [DELTA]HR (before: 324.8[+ or -]6.4 beats/min, after: 336[+ or -]6.2 beats/min).
Microinjection of full-length and truncated Xpress-Egr3DsRed2 does not hinder oocyte maturation
The needle was connected to a 20 [micro]L Hamilton syringe placed on a microinjection pump (CMA/100; BAS, Stockholm, Sweden) with a Teflon tube (0.65 mm OD 9, 0.12 mm OI; Bioanalytical Systems Inc., West Lafayette, IN).
Microinjection method: Microinjection method has been used successfully in the production of transgenic fish and is a commonly used technique due to its simplicity and reliability [7].
Another interesting aspect regarding TA reported in literature is its effectiveness as topical as well as localized intradermal microinjection for melasma.202114 As dose of oral TA in melasma is far less than that prescribed for its hemostatic action
The mRNA format is used for multiplexed screening of gRNA sequences, applications that need delivery by microinjection and easier dosage optimisation of Cas9 protein to gRNA in the cell.
Eppendorf's TransferMan 4r is an innovative micromanipulator that combines an intuitive user interface with enhanced precision for rapid and simple microinjection. The direct transmission of movement in all directions gives a "real-time feeling," and makes the instrument an ideal platform for a range of applications, including microinjection and manipulation of suspension cells such as oocytes and blastocysts.