Activity of fCas9 was proven in animals; for instance, mice bearing mutation on the Bcr locus were generated by microinjection
of fCas9 and gRNAs into fertilized eggs (22).
A novel system consisting in a mold with inserts and heating elements to control the temperature of the surface of a mold for microinjection
molding was developed by De Santis and coworkers [11, 12].
Lim et al., "Localized intradermal microinjection
of tranexamic acid for treatment of melasma in Asian patients: A preliminary clinical trial," Dermatologic Surgery, vol.
As shown in Figures 5(a) and 5(b), microinjection
of baclofen into the NTS induced an elevation in BP in varying degrees in different groups.
(4) RF/DVC+ Va group: the microinjection
of 1microgram remifentanil for 1 [micro]L into the DVC was carried out on the vagotomized rats, followed by the HIR procedure.
of saline (100-150 nL, n=10) into the CnF did not affect [DELTA]MAP (before: 72.1[+ or -]4.59 mm Hg, after: 76.4 3.3 mm Hg) or [DELTA]HR (before: 324.8[+ or -]6.4 beats/min, after: 336[+ or -]6.2 beats/min).
of full-length and truncated Xpress-Egr3DsRed2 does not hinder oocyte maturation
The needle was connected to a 20 [micro]L Hamilton syringe placed on a microinjection
pump (CMA/100; BAS, Stockholm, Sweden) with a Teflon tube (0.65 mm OD 9, 0.12 mm OI; Bioanalytical Systems Inc., West Lafayette, IN).
method has been used successfully in the production of transgenic fish and is a commonly used technique due to its simplicity and reliability .
Another interesting aspect regarding TA reported in literature is its effectiveness as topical as well as localized intradermal microinjection
for melasma.202114 As dose of oral TA in melasma is far less than that prescribed for its hemostatic action
The mRNA format is used for multiplexed screening of gRNA sequences, applications that need delivery by microinjection
and easier dosage optimisation of Cas9 protein to gRNA in the cell.
Eppendorf's TransferMan 4r is an innovative micromanipulator that combines an intuitive user interface with enhanced precision for rapid and simple microinjection
. The direct transmission of movement in all directions gives a "real-time feeling," and makes the instrument an ideal platform for a range of applications, including microinjection
and manipulation of suspension cells such as oocytes and blastocysts.