microinjection

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microinjection

(mī′krō-ĭn-jĕk′shən)
n.
Injection of minute amounts of a substance into a microscopic structure, such as a single cell.

mi′cro·in·ject′ (-jĕkt′) v.

microinjection

A method of introducing DNA into a cell by direct injection into the nucleus, using a microcapillary pipette.

microinjection

a method for introducing new DNA into a CELL by injecting it directly into the NUCLEUS or CYTOPLASM using a thin glass needle. The technique has been used to inject cloned genes into the nucleus of an egg cell in vitro, to generate a TRANSGENIC ORGANISM.
References in periodicals archive ?
Of 112 births from 152 microinjected eggs, only four females and one male were found by researchers to have incorporated the hybrid DNA, or transgene, into their genome.
To determine if sox9b expression levels have an effect on the expression of slincR, we microinjected single-cell zebrafish embryos with a splice-blocking morpholino targeting sox9b (sox9b-MO) or with a control morpholino (Con-MO) and collected RNA at 48 hpf.
To investigate the effect of EA treatment on [GABA.sub.B] receptor function in the NTS, [GABA.sub.B] receptor antagonist CGP-35348 or [GABA.sub.B] receptor agonist baclofen was microinjected into the NTS of rats.
Each rat was microinjected slowly with either 1 [micro]L of NS or 1 fig remifentanil diluted into 1 [micro]L of normal saline into DVC (0.4 millimeter lateral to the midline, 7.9 millimeter below the cranial surface and at occipital crest).
survivin expression in mice transgenic (full-length mouse survivin complementary DNA was cloned and microinjected into C57Bl/6 embryos) [beta]-cells affected neither insulin release nor glucose levels; moreover, there was no difference between wild-type and transgenic mice in islets size and number.
Female BALB/c mice were anaesthetised with 3% isoflurane in 30% [O.sub.2]: 70% [N.sub.2]O, and focally microinjected using a finely drawn glass microcapillary (ca.
After 6 days, five cells per coculture were microinjected with a solution of 5 mg/ml of the membrane-permeant dye LY (Molecular Probes, Eugene, OR, USA) together with 0.5 [micro]g/ml TRITC-dextran 10 kDa (Molecular Probes) by the micromanipulator 5171 and the transinjector 5246 (Eppendorf, Germany).
In this study, NG-nitro-L-arginine methyl ester (L-NAME) (an inhibitor of NO synthase), L-arginine (L-Arg) (a precursor of NO), and sodium nitroprusside (SNP) (a donor of NO) were microinjected into the CnF and cardiovascular responses were investigated.
Such deficits can occur as a result of brain trauma during the microinjection procedure, but we can exclude this possibility because a group microinjected with saline showed similar performance as a blank group that was not microinjected.
Egr3 siRNA (25 [micro]M) was microinjected into GV oocytes and rates of GVBD and polar body extrusion were monitored.
In these procedures, ovaries are down-regulated and then stimulated to produce eggs, which retrieved, and then microinjected with spermatozoa.