Of 112 births from 152 microinjected
eggs, only four females and one male were found by researchers to have incorporated the hybrid DNA, or transgene, into their genome.
To determine if sox9b expression levels have an effect on the expression of slincR, we microinjected
single-cell zebrafish embryos with a splice-blocking morpholino targeting sox9b (sox9b-MO) or with a control morpholino (Con-MO) and collected RNA at 48 hpf.
To investigate the effect of EA treatment on [GABA.sub.B] receptor function in the NTS, [GABA.sub.B] receptor antagonist CGP-35348 or [GABA.sub.B] receptor agonist baclofen was microinjected
into the NTS of rats.
Each rat was microinjected
slowly with either 1 [micro]L of NS or 1 fig remifentanil diluted into 1 [micro]L of normal saline into DVC (0.4 millimeter lateral to the midline, 7.9 millimeter below the cranial surface and at occipital crest).
survivin expression in mice transgenic (full-length mouse survivin complementary DNA was cloned and microinjected
into C57Bl/6 embryos) [beta]-cells affected neither insulin release nor glucose levels; moreover, there was no difference between wild-type and transgenic mice in islets size and number.
Female BALB/c mice were anaesthetised with 3% isoflurane in 30% [O.sub.2]: 70% [N.sub.2]O, and focally microinjected
using a finely drawn glass microcapillary (ca.
After 6 days, five cells per coculture were microinjected
with a solution of 5 mg/ml of the membrane-permeant dye LY (Molecular Probes, Eugene, OR, USA) together with 0.5 [micro]g/ml TRITC-dextran 10 kDa (Molecular Probes) by the micromanipulator 5171 and the transinjector 5246 (Eppendorf, Germany).
In this study, NG-nitro-L-arginine methyl ester (L-NAME) (an inhibitor of NO synthase), L-arginine (L-Arg) (a precursor of NO), and sodium nitroprusside (SNP) (a donor of NO) were microinjected
into the CnF and cardiovascular responses were investigated.
Such deficits can occur as a result of brain trauma during the microinjection procedure, but we can exclude this possibility because a group microinjected
with saline showed similar performance as a blank group that was not microinjected
The targeted ES cells were microinjected
Egr3 siRNA (25 [micro]M) was microinjected
into GV oocytes and rates of GVBD and polar body extrusion were monitored.
In these procedures, ovaries are down-regulated and then stimulated to produce eggs, which retrieved, and then microinjected