found a 5.9% of T vivax infections in bovines ([n.sub.T]=1572), determined by the microhematocrit
technique and 33.1% seropositivity through IFAT and ELISA ([n.sub.T]=1675) in forty-nine Venezuelan farms.
True Hgb = measured Hgb--[Hgb of patient's lipemic plasma sample x (1 - microhematocrit
A thin capillary tube called a microhematocrit
tube is filled with blood and sealed at the bottom.
Packed cell volume (PCV) of samples was determined using microhematocrit
centrifuge at 12000 g for 5min ; white blood cell (WBC) count and differentials were determined by mechanical expansion and optical magnification, augmented by supravital cell staining .
Exercises that come under this category include reporting of peripheral smear and / or bone marrow smears provided with relevant clinical data and basic hemogram; reading, interpretation and correlation of ESR, microhematocrit
, reticulocyte count, osmotic fragility.
For Hct determination, capillary tubes were centrifuged for five minutes in a microhematocrit
centrifuge (Damon/IEC), and Hct was determined using a microcapillary tube reader (Damon/IEC).
Haematocrit (Ht) values were determined using the microhematocrit
method and hemoglobin (Hb) levels and erythrocyte number (E) were measured using the cyanmethemoglobin colorimetric method outlined in the Sigma assay kit (Sigma Diagnostics).
PCV was determined by using microhematocrit
method (6) using microhematocrit
centrifuge and reader (Hawksley, England), expressed in percents (%).
Globular volume for the microhematocrit
method was performed using a personal computer EULAB, model 024.
INDEX TERMS: capillary tube; hematocrit; microhematocrit
A wet preparation of the buffy coat area of a microhematocrit
tube after centrifugation may show motile organisms.