Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array
Among the available approaches, a microelectronic array technology has been developed for DNA hybridization analysis of mutations/ single-nucleotide polymorphisms (SNPs) (1-4).
Active microelectronic array system for DNA hybridization, genotyping and pharmacogenomic applications.
Determination of the factor V Leiden single- nucleotide polymorphism in a commercial clinical laboratory by use of NanoChip microelectronic array technology.
Alternatives include bead array technologies (39, 40) and microelectronic array devices (41, 42), which yield information in seconds or minutes rather than hours or days, that allow for rapid estimation of gene expression.
Microelectronic array devices and techniques for electric field enhanced DNA hybridization in low-conductance buffers.
Microelectronic array technology was recently introduced for SNP analysis (11-15).
The protocols we present should provide a vehicle to enable the potential of microelectronic array methods to be realized more quickly in a clinical laboratory setting, facilitating high-throughput, cost-effective molecular diagnostic testing of genetic variations as well as mutation detection.
Pathogen analysis and genetic predisposition testing using microelectronic arrays and isothermal amplification.
Alternatively, the method we describe is highly accurate and uses automated microelectronic array technology analysis.
The NanoChip microelectronic array technology for analysis of the FVL SNP provides extremely high accuracy, readily discriminating between wild types, heterozygotes, and homozygotes, as confirmed by comparison with DNA sequencing.