The microbroth dilution method
was applied to determine minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of F1 and F2 mouthwashes and essential oil samples.
We performed antifungal susceptibility testing for amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, caspofungin, anidulafungin, and micafungin by the microbroth dilution method
recommended by the Clinical and Laboratory Standards Institute (10).
Determination of MIC value for the active antimicrobial fraction was determined by Microbroth dilution method
Antifungal susceptibility test for the Candida isolates was done by Microbroth dilution method
, as per the guidelines of Clinical and Laboratory Standard Institutes (CLSI) on antifungal Susceptibility testing .
Detection of MIC by microbroth dilution method for oxacillin: Oxacillin, (Sigma-Aldrich Company, USA) stock solution was prepared at concentrations of 1280 [micro]g/ml in deionized water.
By microbroth dilution method, of the 110 staphylococcal isolates, 8 (7.2%) had MIC of 32 [micro]g/ml by oxacillin, 22 (20%) had 16 [micro]g/ml, 10 (9.1%) each had 8, 4 and <2 [micro]g/ml.
Susceptibility testing of the initial blood and urine isolates was performed by using the radiometric broth MIC method (Becton Dickinson BACTEC 460 radiometric system; Becton Dickinson Microbiology Systems) and by microbroth dilution method
(TREK Diagnostics, Cleveland, OH, USA) for isoniazid and streptomycin.
In this study we assessed the influence of glucose added urine on the in vitro activities of various antibiotics by the microbroth dilution method.
Minimum inhibitory concentrations (MICs) were performed by the microbroth dilution method parallel in Mueller Hinton broth and glucose added urine.
For telithromycin, because an E-test strip was unavailable, a microbroth dilution method was used.
To understand the possible resistance mechanism, MICs for 2 lincosamides (clindamycin and lincomycin) were determined by using a microbroth dilution method in the presence and absence of 10 mg/L of the efflux pump inhibitor reserpine (Sigma Chemicals, St Louis, MO, USA), as described (7); S.
By the microbroth dilution method
(6), the HF-1 isolate was sensitive to clindamycin, clarithromycin, azithromycin, erythromycin, tetracycline, doxycycline, ofloxacin, and chloramphenicol but resistant to vancomycin and gentamicin.