Comparison of different phenotypic tests for detection of MBL producing isolates Phenotypic test Number of MBL positive isolates (n=21) Modified Hodge test on Mueller Hinton Agar 15 Modified Hodge test on MacConkey Agar 19 Combined Ceftazidime + EDTA 8 disc test Imipenem + EDTA 20 Double disc synergy test 20 EDTA-Imipenem microbiological assay
20 Extended EDTA Ceftazidime 12 disc synergy Imipenem 20 test Meropenem 18 n, number of screen positive isolates tested
Whole-blood folate values in subjects with different methylenetetrahydrofolate reductase genotypes: differences between the radio-assay and microbiological assays
for vitamin B12 performed in 96-well microtitre plates.
A microbiological assay
has commonly been used to analyze the amount of B12 in samples, but that method takes days and is expensive.
Serum cobalamin was determined by a Lactobacillus leichmannii microbiological assay
(19) and serum and wholeblood folate by a L.
We recently combined the advantages of a microbiological assay
and DBS in an assay for erythrocyte folate (1).
These EDTA-WB hemolysates require a further 20-fold (or more) dilution before analysis by microbiological assay
with Lactobacillus rhamnosus.
casei), used in the classical microbiological assay
of folate, would prevent the saponin molecule from reaching the outer bacterial membrane.