microarray analysis


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Related to microarray analysis: Microarray technology, DNA microarrays, CDNA microarray

microarray analysis

A computerised, robotic technology used to rapidly analyse mutated or missing genes. Microarray analysis provides a way of simultaneously studying the interaction of a large numbers of genes with each other and with regulatory networks that control batteries of genes.
 
Method
A robot precisely applies droplets containing functional DNA to glass slides; fluorescent labels are then attached to the DNA from the cell being studied. The labelled probes are allowed to hybridise to cDNA strands on the slides, which are then placed into a scanning microscope that can measure the fluorescence of each dot, the brightness of which serves to quantify the amount of a specific DNA fragment.
References in periodicals archive ?
A-C, Blood serum samples were derived from those used for microarray analysis (n=3 for each group).
qRT-PCR was performed to verify DEGs determined in through microarray analysis. Briefly, TRIzol reagent (Invitrogen) was used to extract total tissue sample RNA followed by cDNA synthesis utilizing PrimeScript RT reagents (Takara Bio Inc.).
To validate the changes in miRNA expression detected by microarray platform, qRT-PCR analyses using RNA from both dependent (original RNA extracted from the young normal and old OA donors used in microarray analysis) and independent cohorts were performed.
* chromosomal microarray analysis versus karyotype for specific clinical situations
Total RNA from nrf2a-overexpressing embryos and DEM-treated larvae was prepared according to the procedure that was performed in the microarray analysis. cDNA was synthesized using ReverTra Ace qPCR RT Master Mix (Toyobo, Osaka, Japan) according to the manufacturer's instructions.
(7) In addition, labs detecting mutations and CNVs utilizing NGS should use a secondary technology such as Sanger sequencing and microarray analysis or qPCR, respectively, in order to eliminate the potential of reporting false positives.
Four components of evaluation--autopsy, placental examination, karyotype or microarray analysis, and fetal-maternal hemorrhage testing--have proven to be high-yield tests when performed in all cases of stillbirth.
Standard microarray analysis was performed on peripheral whole blood samples taken before and after each treatment.
The same protein microarray analysis of ASC-CM was used in both articles as Figures 2(a) and 6(a), respectively, but different cytokines were marked in the same protein microarray picture.
Although aCGH and SNP microarray analysis have numerous advantages, several technical and analytical limitations exist (14).