methyl red test


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Related to methyl red test: nitrate reduction test, urease test

methyl red test

A colorimetric test for differentiating Enterobacteriaceae.

Method
An inoculum of bacteria is placed in a buffered glucose-peptone broth; the MRT is based on a change in pH, and usually results in the opposite reaction as that of the Voges-Proskauer test—therefore Enterobacter aerogenes, which is positive in the VPT, serves as a negative control in the MRT. Escherichia coli is positive (red) at 48 hours, Enterobacter aerogenes is negative.
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Development of red colour was taken as positive and development of yellow colour was taken as negative for methyl red test.
Characteristic of other Bacterial isolates from Amirthi forest Biochemical VITNJ1 VITNJ2 VITNJ3 VITNJ4 VITNJ5 Properties Gram Staining + + + + + Shape Rod Rod Rod Rod Rod Indole test - - - - - Methyl red test - - + + - Voges Proskauer + + + + - Citrate Utilization + + + + + Oxidase test - - + + - Catalase Test - - + + + Urease - - - - - Biochemical VITNJ6 VITNJ7 VITNJ8 VITNJ9 Properties Gram Staining + + - + Shape Rod Rods Rods Rods Indole test - + - - Methyl red test - - - + Voges Proskauer - - + + Citrate Utilization - - + - Oxidase test - - - + Catalase Test + + + + Urease - - - - Table 3.