It was found to be positive for glucose, lactose and sucrose fermentation (red colour appeared in test tube) but found to be negative for catalase test (no bubbles were formed), indole test (green layer is formed at the top of test tube), nitrate reduction test, citrate test (green-forest colour in test tube), methyl red
Voges-Proskeur test (no red colour appears in test tube), casein hydrolysis test and for starch agar test (no clear zone formation) as shown in Table 1.
Aerobic conditions and the dynamics of the methyl red
reaction in Klebsielleae.
The indicator, two drops of methyl red
indicator, was added just before the start of the titration.
Positive results for catalase and methyl red
tests and negative results for oxidase, indole utilization tests and Voges-Proskauer tests were recorded.
Their growth on Eosin methylene blue agar with appearance of green metallic sheen, positive for indole and oxidase production, methyl red
test and negative Voges-Proskauer and citrate utilization tests confirmed these isolates as those of E.
Conventional test were performed by bacterial culture on: Indole (BD), Citrate Simmons (Oxoid), Voges Proskauer (BD), Triple Sugar-Iron (BD), Urea (BD), Motility, Methyl Red
(Oxoid) and gas production (BD) (11).
In a MFC, 106 mWcm-2 current density was generated which increased to 207 mWcm-2 and 164 mWcm-2with introduction of mediator methyl red
and methyl orange respectively .The decrease in solution resistance Rs was observed from 410 a| to 282 a| with the addition of methyl orange and to 153 a| with methyl red
On the basis of biochemical characterization the five isolates were found to be non-motile, also gave positive result for nitrate reduction test and methyl red
test, while negative result was obtained for indole test.
Methyl orange, methyl red
and Congo red are the dyes being used as acid base indicators because of their ability to act as weak acids or bases.
Hsueh and Chen (2007) reported MR as the most difficult dye to degrade due to charged carboxyl group on Methyl red
at ortho position to azo bond, inhibiting decolorization activity.
Radiation induced color bleaching of an azo dye; called methyl red
in poly (vinyl butyral) has been examined as a high dose dosimeter (12).