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Methyl green, a dye that stains phosphorylated polysaccharides in onuphid polychaetes (Winsnes, 1985), labeled cement cells (Fig.
The use of Methyl Green as an aid in species discrimination in Onuphidae (Annelida, Polychaeta).
This supposition is supported by the fact that nuclear staining with methyl green solution or ethidium bromide became clearly decreased in the remaining histologic sections after the antigen-retrieval treatment, suggesting a loss of nuclear chromatin due to heating (data not shown).