and PAS staining for fungal organism, Ziehl-Neelsen staining, and PCR for Mycobacterium tuberculosis were all negative.
Special staining by Grocott-Gomori methenamine-silver
stain of transbronchial and thoracic lymph node biopsy specimens from a hospitalized student revealed the presence of yeasts consistent with H.
The standard method using a Grocott-Gomori methenamine-silver
stain is easy and reliable.
al., 2001); using special stains H & E (hematoxylineosin) or methenamine-silver
stain (Pirani, 1996).
Stains for acid-fast bacilli with Grocott-Gomori methenamine-silver
nitrate were negative.
The Grocott-Gomori methenamine-silver
nitrate stain will allow the visualisation of chains of darkly pigmented, spheroidal, yeast-like organisms.
(6) A variety of stains, including H & E, Grocott-Gomori methenamine-silver
nitrate, and PAS frequently reveal characteristic hyphal elements in tissue.
The histochemical stain results for acid-fast bacilli (Ziehl-Neelsen) and fungi (Gomori methenamine-silver
stain) were likewise negative.
Tissues were sectioned in 5-[micro]n slices and were subsequently stained with hematoxylin and eosin, Gram's, Ziehl-Neelsen, periodic acid--Schiff, and Grocott methenamine-silver
D) Grocott's methenamine-silver
stain showing broad-based budding yeast.
Caption: Figure 3: Grocott-Gomori methenamine-silver
(GMS) stained tissue section of resected lung from an 18-year-old with recent kidney transplant highlighting aseptate hyphae morphologically resembling fungal class Zygomycetes (400x).