Aspergillus spp can also be visualized with Papanicolaou stain with a similar appearance to that with Grocott methenamine silver stain
(Papanicolaou, original magnification X400).
Inset: Stain highlighting black fungal hyphae and conidiophore embedded within eosinophilic debris in the mucosa (Gomori methenamine silver stain
Some nonsclerotic glomeruli were accompanied by fibrocellular crescents (b) ((a), periodic acid methenamine silver stain
, original magnification 400x; (b), periodic acid-Schiff stain, original magnification 400x).
Methenamine silver stain
showed filamentous bacteria-like structures staining positive on gram stain which were weakly positive on the Fite staining method, supporting a diagnosis of Nocardia renal abscess (Figure 3).
Fruiting bodies (conidiophores) were visible, including the stalk (variably present), central vesicle with circumferentially biseriate, radiating linear metulae and phialides, and peripheral round, pigmented conidia (Figures 4(a) and 4(b)) and Grocott's methenamine silver stain
rarely demonstrated septae.
Paraffin sections of the skin, oral mucosa, duodenum, pancreas and mesenteric lymph nodes were stained with Grocott's methenamine silver stain
(GMS) for the identification of Pythium insidiosum.
Slices were stained with haematoxylineosin (HE), Masson, and methenamine silver stain
(PASM), respectively, and visualized using light microscopy.
The cyst walls are thick and birefringent, stained with hematoxylin and eosin (deeply magenta), Grocott Gomori methenamine silver stain
(GMS), digested periodic acidSchiff, and Mayer mucicarmine.
Gommori's methenamine silver stain
was positive (Fig: 6).
Hence, special stains like Periodic acid Schiff stain (PAS) and Grocott's methenamine silver stain
(GMS) were used, which showed occasional septate fungal hyphae (Figure 1, arrow).
4), Gomori's methenamine silver stain
showed branching slender fungal hyphae (Fig.