Therefore, a thorough preoperative clinical assessment and examination of the nonneoplastic portion of tumor nephrectomy specimens using the recommended special stain (PAS or Jones methenamine
silver) or other studies (immunohistochemistry/electron microscopy) if necessary may provide valuable diagnostic and prognostic information for appropriate management.
A total of 486 samples were sent to the Microbiology Lab during this period for Silver methenamine
Dissected out specimens were subjected to routine histological processing (Mc Manus and Mowry 1960).The paraffin blocks thus obtained were serially sectioned at a thickness of 10 [micro]m for staining with Haematoxylin and Eosin and 20-25 [micro]m thickness for special stains like Silver Methenamine
staining to demonstrate the nerve fibres.
A biopsy of left deltoid and both vastus lateralis muscles showed necrotizing granulomatous inflammation without any organisms by Gram, acid-fast bacilli, Wright, and Gomori methenamine
silver and Gram stain results were negative in lung sections.
Neither PAS nor Gomori methenamine
silver staining revealed any fungal hyphae, and fungal cultures were negative.
Muscle tissue after fixation was stained with hematoxylin and eosin, periodic acid-Schiff, mucicarmine, Grocott methenamine
silver, Giemsa, Warthin-Starry silver, acid-fast, and Lillie-Twort Gram stains.
To further compare the results of MMPS versus other stains traditionally used for tissue identification of MCC, representative study cases containing MCC or talc, and the 2 cases of mixed silicate or oxalate deposition were also stained using Gomori methenamine
silver and Congo red stains.
The acidfast stain for mycobacteria and the Gomori methenamine
silver stain were negative.
Large, ovoid yeast cells with doublecontoured walls, [approximately equal to] 10-15 [micro]m, were identified in this exudate by using the Grocott methenamine
silver staining technique and IHC for B.
There are 2 main patterns of glomerular solidification (Figure 1): In most primary and secondary glomerular diseases, glomeruli undergo matrix overproduction or impaired matrix degradation and turn globally sclerotic with material that is positive for periodic acid-Schiff (PAS) and methenamine
silver occupying most of the Bowman space.
Findings at the light microscopic level enhanced by data obtained from the examination of special stains (periodic acid-Schiff, methenamine
silver, and trichrome stains) generally become the first source of information and can guide the diagnostic investigation in the right direction.